PMID: 9185164Jul 1, 1997Paper

Characterization of a nuclear factor that enhances DNA binding activity of SSCRE-BP/PUR alpha, a single-stranded DNA binding protein

Neurochemistry International
Y DingN Miki

Abstract

Pur alpha has been identified as a single-stranded DNA binding protein that specifically binds to the purine-rich strand present in the DNA replication initiation zone of the human c-myc gene. We have previously demonstrated that chronic morphine treatment decreases the DNA binding activity of ssCRE-BP (single-stranded cyclic AMP response element-binding protein), which has been shown to be identical to pur alpha by cDNA cloning, and is abundant in the brain. In this report we identified an activator of ssCRE-BP/pur alpha in the brain and characterized it. Although purified ssCRE-BP/pur alpha or its GST-fusion protein exhibited very low DNA binding activities, they were markedly enhanced by including nuclear extract in the binding assay. The enhanced binding activity is trypsin-sensitive, heat-stable and has a molecular weight of approximately 66 kDa. Casein could substitute for the activator and increased the DNA binding activity of ssCRE-BP/pur alpha by one order. A series of deletion mutants were prepared in order to determine the DNA binding and activator interacting domains, and both of them were found to reside in AA 50-215 of ssCRE-BP/pur alpha. These data suggest that the DNA binding activity of ssCRE-BP/pur alpha is au...Continue Reading

References

Dec 11, 1992·Nucleic Acids Research·Y HeraultD Michel
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M T MurrayW W Franke
Jan 26, 1990·Cell·A A Travers
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M R MontminyR H Goodman
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E J LewisD M Chikaraishi
Sep 1, 1986·Nature·M CombH M Goodman
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·W H WalkerJ F Habener
Feb 24, 1995·The Journal of Biological Chemistry·G H MacDonaldJ P Ting
Mar 1, 1993·DNA and Cell Biology·J S WilliamsO M Andrisani

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Citations

Nov 9, 2000·Molecular and Biochemical Parasitology·M R FantappieP T LoVerde
Aug 23, 2000·Nucleic Acids Research·G L GalliaK Khalili
Aug 28, 2007·Proteomics·Julia StrathmannMichael Schwarz
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·John J MaddenRobert M Donahoe
Nov 24, 2006·The Journal of Biological Chemistry·Jon E RamseyRobert J Kelm
Mar 30, 2021·Frontiers in Genetics·Lena MolitorDierk Niessing

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