PMID: 9428689Jan 15, 1998Paper

Characterization of a nuclear localization signal of canine parvovirus capsid proteins

European Journal of Biochemistry
Maija Vihinen-RantaM Vuento

Abstract

We investigated the abilities of synthetic peptides mimicking the potential nuclear localization signal of canine parvovirus (CPV) capsid proteins to translocate a carrier protein to the nucleus following microinjection into the cytoplasm of A72 cells. Possible nuclear localization sequences were chosen for synthesis from CPV capsid protein sequences (VP1, VP2) on the basis of the presence of clustered basic residues, which is a common theme in most of the previously identified targeting peptides. Nuclear targeting activity was found within the N-terminal residues 4-13 (PAKRARRGYK) of the VP1 capsid protein. While replacement of Arg10 with glycine did not affect the activity, replacement of Lys6, Arg7, or Arg9 with glycine abolished it. The targeting activity was found to residue in a cluster of basic residues, Lys5, Arg7, and Arg9. Nuclear import was saturated by excess of unlabelled peptide conjugates (showing that it was a receptor-mediated process). Transport into the nucleus was an energy-dependent and temperature-dependent process actively mediated by the nuclear pores and inhibited by wheat germ agglutinin.

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Citations

Jun 6, 2003·Advanced Drug Delivery Reviews·Gary R Whittaker
Aug 15, 2008·Journal of Virology·Christian D S NelsonColin R Parrish
Oct 22, 2002·Journal of Virology·Wu XiaoNicholas Muzyczka
Sep 13, 2003·Journal of Virology·Sanna SuikkanenMaija Vihinen-Ranta
Jun 15, 2004·Journal of Virology·Maija Vihinen-RantaColin R Parrish
Oct 14, 2000·Annual Review of Cell and Developmental Biology·G R WhittakerA Helenius
Nov 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Glen A FarrPeter Tattersall
Oct 30, 2010·Journal of Molecular and Cellular Cardiology·Jinhui WangJoseph E Rabinowitz
Apr 15, 2008·Trends in Microbiology·Carole E HarbisonColin R Parrish
Mar 10, 2016·Annual Review of Virology·Shweta KailasanColin R Parrish
Jun 15, 2012·The Journal of General Virology·Ruth Popa-WagnerJürgen A Kleinschmidt
Dec 21, 2010·Biochimica Et Biophysica Acta·Sarah CohenNelly Panté
Aug 5, 2015·Virology Journal·Mengyu TuAnchun Cheng
Jun 2, 2015·Frontiers in Microbiology·Nikta Fay, Nelly Panté
Oct 26, 2013·Advances in Biological Regulation·Kenza Snoussi, Michael Kann
Jan 3, 2009·Biochemical and Biophysical Research Communications·Kirsi PakkanenMatti Vuento
Dec 3, 2003·Virology·Sanna SuikkanenMatti Vuento
May 4, 2010·PLoS Neglected Tropical Diseases·Claudia BönschCarlos Ros
Sep 4, 2007·Advances in Virus Research·Susan F Cotmore, Peter Tattersall
Oct 5, 2017·Viruses·Elina MäntyläMaija Vihinen-Ranta
Oct 27, 2017·Viruses·Carlos RosJosé M Almendral
Nov 22, 2019·Journal of Virology·Elina MäntyläMaija Vihinen-Ranta
Apr 16, 2017·Virology Journal·Peng LiuAnchun Cheng
Oct 13, 2006·The Journal of General Virology·Sarah CohenNelly Panté

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