Characterization of a nucleoside/proton symporter in procyclic Trypanosoma brucei brucei.

The Journal of Biological Chemistry
Harry P De KoningS M Jarvis

Abstract

Adenosine transport at 22 degrees C in procyclic forms of Trypanosoma brucei brucei was investigated using an oil-inhibitor stop procedure for determining initial rates of adenosine uptake in suspended cells. Adenosine influx was mediated by a single high affinity transporter (Km 0.26 +/- 0.02 microM, Vmax 0.63 +/- 0.18 pmol/10(7) cells s-1). Purine nucleosides, with the exception of tubercidin (7-deazaadenosine), and dipyridamole inhibited adenosine influx (Ki 0.18-5.2 microM). Purine nucleobases and pyrimidine nucleosides and nucleobases had no effect on adenosine transport. This specificity of the transporter appears to be similar to the previously described P1 adenosine transporter in bloodstream forms of trypanosomes. Uptake of adenosine was Na+-independent, but ionophores reducing the membrane potential and/or the transmembrane proton gradient (monitored with the fluorescent probes bis-(1,3-diethylthiobarbituric acid)-trimethine oxonol and 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein acetoxymethyl ester, respectively) inhibited adenosine transport. Similarly, an increase in extracellular pH from 7.3 to 8.0 reduced adenosine influx by 30%. A linear correlation was demonstrated between the rate of adenosine transport and ...Continue Reading

References

Jul 1, 1992·The Biochemical Journal·G O Gbenle, D M Dwyer
Apr 1, 1991·Experimental Parasitology·J A Thissen, C C Wang
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M GottliebD M Dwyer
Jan 2, 1987·Molecular and Biochemical Parasitology·B AronowB Ullman
Jun 1, 1972·Bacteriological Reviews·F M Harold
Mar 1, 1982·Journal of Medicinal Chemistry·J W Daly
Nov 1, 1984·Molecular and Biochemical Parasitology·D J Hammond, W E Gutteridge
Oct 1, 1980·Parasitology·D M James, G V Born
Mar 1, 1995·Molecular and Biochemical Parasitology·J C SchwabK A Joiner
Feb 1, 1995·Molecular and Biochemical Parasitology·B J BergerA H Fairlamb
Jan 1, 1994·Annual Review of Microbiology·D Zilberstein, M Shapira
Mar 10, 1995·The Journal of Biological Chemistry·L VieiraZ I Cabantchik
Jan 1, 1993·Advances in Parasitology·D Zilberstein
Dec 1, 1993·International Journal for Parasitology·S T HallA M Gero
Sep 1, 1993·The Journal of Eukaryotic Microbiology·K F BaumJ J Marr
Apr 1, 1993·The International Journal of Biochemistry·P O Ogbunude, H P Baer
Jun 1, 1996·General Pharmacology·J A Thorn, S M Jarvis
Oct 29, 1996·Biochimica Et Biophysica Acta·D A Griffith, S M Jarvis
Nov 19, 1997·Molecular and Biochemical Parasitology·H P de Koning, S M Jarvis

❮ Previous
Next ❯

Citations

Dec 4, 2003·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Pascal MäserRonald Kaminsky
May 4, 2001·International Journal for Parasitology·H P de Koning
Jul 17, 2003·International Journal for Parasitology·Harry P De KoningJonathan M Wastling
Sep 3, 2003·Pharmacology & Therapeutics·Mahmoud H el Kouni
May 10, 2000·Molecular and Biochemical Parasitology·M Ghosh, T Mukherjee
Aug 30, 2003·Molecular and Biochemical Parasitology·Cristina HenriquesScott M Landfear
Apr 1, 2000·Molecular and Biochemical Parasitology·H P de KoningS M Jarvis
Sep 18, 2003·FEMS Microbiology Letters·Philippe LawtonAnne Françoise Petavy
Dec 22, 1999·Trends in Microbiology·N S CarterH P de Koning
May 14, 1999·Molecular Medicine Today·S A BaldwinJ D Young
Apr 5, 2001·Trends in Parasitology·N S CarterB Ullman
May 24, 2003·Trends in Parasitology·Patrick G BrayHarry P de Koning
Mar 3, 2010·Antimicrobial Agents and Chemotherapy·Maya BergKoen Augustyns
Jun 15, 2007·Eukaryotic Cell·Daniel C Turnock, Michael A J Ferguson
Oct 14, 2003·Eukaryotic Cell·Enock MatovuHarry P de Koning
Apr 13, 2004·Eukaryotic Cell·Scott M LandfearMarco A Sanchez
Mar 3, 2009·Bioorganic & Medicinal Chemistry·Constance CholletIan H Gilbert
Nov 26, 2008·Experimental Parasitology·Milane S LeiteJosé Roberto Meyer-Fernandes
Dec 5, 2006·Experimental Parasitology·Milane de Souza LeiteJosé Roberto Meyer-Fernandes
Apr 20, 2006·Experimental Parasitology·Simon GudinHarry P de Koning
Mar 7, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Alexandra LüscherPascal Mäser
Jul 10, 2010·Bioorganic & Medicinal Chemistry Letters·Nina KleeIan H Gilbert
Feb 13, 2001·Molecular Phylogenetics and Evolution·L I GrossmanM Goodman
Jul 26, 2005·FEMS Microbiology Reviews·Harry P de KoningRichard J S Burchmore
Feb 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Jane C MundayHarry P de Koning
May 25, 2007·Molecular and Biochemical Parasitology·Iris SpoerriGabriela Burkard
Dec 1, 2006·Advances in Parasitology·Michael P Barrett, Ian H Gilbert
Mar 6, 2009·Organic & Biomolecular Chemistry·Alessandro BalianiIan H Gilbert
Jul 3, 2009·Clinical Pharmacology and Therapeutics·S M Landfear
Jun 14, 2006·The Journal of Biological Chemistry·Raquel ValdésScott M Landfear
Feb 6, 2014·The Journal of Biological Chemistry·Raquel ValdésScott M Landfear

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.