Characterization of a Pachymedusa dacnicolor-Sauvagine analog as a new high-affinity radioligand for corticotropin-releasing factor receptor studies

The Journal of Pharmacology and Experimental Therapeutics
M H PerrinP E Sawchenko

Abstract

The corticotropin-releasing factor (CRF) peptide family comprises the mammalian peptides CRF and the urocortins as well as frog skin sauvagine and fish urophyseal urotensin. Advances in understanding the roles of the CRF ligand family and associated receptors have often relied on radioreceptor assays using labeled CRF ligands. These assays depend on stable, high-affinity CRF analogs that can be labeled, purified, and chemically characterized. Analogs of several of the native peptides have been used in this context, most prominently including sauvagine from the frog Phyllomedusa sauvageii (PS-Svg). Because each of these affords both advantages and disadvantages, new analogs with superior properties would be welcome. We find that a sauvagine-like peptide recently isolated from a different frog species, Pachymedusa dacnicolor (PD-Svg), is a high-affinity agonist whose radioiodinated analog, [(125)ITyr(0)-Glu(1), Nle(17)]-PD-Svg, exhibits improved biochemical properties over those of earlier iodinated agonists. Specifically, the PD-Svg radioligand binds both CRF receptors with comparably high affinity as its PS-Svg counterpart, but detects a greater number of sites on both type 1 and type 2 receptors. PD-Svg is also ∼10 times more ...Continue Reading

References

Aug 1, 1981·International Journal of Peptide and Protein Research·P C Montecucchi, A Henschen
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·J GulyasJ Rivier
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·E PotterW Vale
Dec 31, 1997·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·R J PrimusD W Gallager
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·A RühmannJ Spiess
Feb 2, 2000·Biochemistry·P M KassonH M McConnell
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M NielsenW W Vale
Nov 7, 2000·The Journal of Comparative Neurology·K Van PettP E Sawchenko
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·T M ReyesP E Sawchenko
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·K LewisW W Vale
Jan 12, 2002·Current Opinion in Pharmacology·Johannes M H M Reul, Florian Holsboer
Aug 28, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Alexandra WiggerRainer Landgraf
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Tracy L Bale, Wylie W Vale
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christy R R GraceRoland Riek
Mar 1, 2005·European Journal of Pharmacology·Donald R GehlertTammy J Sajdyk
Aug 22, 2006·Peptides·Beatrice WaserJean Claude Reubi
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christy Rani R GraceRoland Riek
Sep 24, 2013·Biochimica Et Biophysica Acta·Rajashri SridharanMark E Dumont
Nov 26, 2013·Frontiers in Neuroendocrinology·Jean E Rivier, Catherine L Rivier
Apr 1, 2014·Current Opinion in Cell Biology·James W WislerRobert J Lefkowitz

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Citations

Jan 21, 2016·Journal of Medicinal Chemistry·Judit ErchegyiJean E Rivier
Sep 9, 2017·Journal of Forensic Sciences·Isabella AquilaPietrantonio Ricci
Aug 16, 2018·Physiological Reviews·Jan M Deussing, Alon Chen

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