Characterization of a Par j 1/Par j 2 mutant hybrid with reduced allergenicity for immunotherapy of Parietaria allergy

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
A BonuraP Colombo

Abstract

Parietaria pollen is one of the major cause of pollinosis in the southern Europe. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease restoring a normal immunity against allergens. We designed a recombinant molecule (PjEDloop1) comprised of genetic-engineered variants of the major allergens of the Parietaria pollen (Par j 2/Par j 1). Purity and chemical-physical properties of the derivative were analysed by RP-HPLC chromatography and Photon Correlation Spectroscopy. Immunological activity was evaluated by means of Western blotting, ELISA inhibition and PBMC proliferation assay in 10 Parietaria allergic patients. Basophil activation was studied in six subjects. The immunogenicity of the hybrid was studied looking at the immune responses induced in a mouse model of sensitization. The PjEDloop1 hybrid was produced as a purified recombinant protein with high stability in solution. Western blot, ELISA inhibition and basophil activation test showed that the PjEDloop1 displays a remarkable reduced IgE binding and anaphylactic activity. CD3 reactivity was conserved in all patients. Mice immunization with the rPjEDloop1 induced antibodies and T cell responses comparable to that obtained by the ...Continue Reading

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Citations

Nov 29, 2013·Immunotherapy·Michael WallnerFatima Ferreira
Oct 7, 2014·Molecular Immunology·Valeria LongoPaolo Colombo
Oct 15, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A MariM Wickman
Dec 11, 2013·Clinical & Developmental Immunology·C Gómez-CasadoL Tordesillas
Feb 5, 2014·International Journal of Pharmaceutics·Mariano LicciardiPaolo Colombo
Jun 29, 2013·Methods : a Companion to Methods in Enzymology·Gabriele GadermaierFatima Ferreira
Oct 30, 2016·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Angelica TiotiuPascal Poncet
Jun 27, 2019·International Journal of Molecular Sciences·Dalgys MartínezLeonardo Puerta
Dec 14, 2018·Medicina·Giorgio CiprandiSimonetta Masieri

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