Characterization of a partially folded monomer of the DNA-binding domain of human papillomavirus E2 protein obtained at high pressure.

The Journal of Biological Chemistry
D FoguelGonzalo de Prat-Gay

Abstract

The pressure-induced dissociation of the dimeric DNA binding domain of the E2 protein of human papillomavirus (E2-DBD) is a reversible process with a Kd of 5.6 x 10(-8) M at pH 5.5. The complete exposure of the intersubunit tryptophans to water, together with the concentration dependence of the pressure effect, is indicative of dissociation. Dissociation is accompanied by a decrease in volume of 76 ml/mol, which corresponds to an estimated increase in solvent-exposed area of 2775 A2. There is a decrease in fluorescence polarization of tryptophan overlapping the red shift of fluorescence emission, supporting the idea that dissociation of E2-DBD occurs in parallel with major changes in the tertiary structure. The dimer binds bis(8-anilinonaphthalene-1-sulfonate), and pressure reduces the binding by about 30%, in contrast with the almost complete loss of dye binding in the urea-unfolded state. These results strongly suggest the persistence of substantial residual structure in the high pressure state. Further unfolding of the high pressure state was produced by low concentrations of urea, as evidenced by the complete loss of bis(8-anilinonaphthalene-1-sulfonate) binding with less than 1 M urea. Following pressure dissociation, a pa...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
Nov 1, 1991·Trends in Biochemical Sciences·J HamM Yaniv
Jan 1, 1990·Annual Review of Biochemistry·P S Kim, R L Baldwin
Sep 5, 1989·Biochemistry·J U Bowie, R T Sauer
Apr 1, 1987·Biophysical Journal·J R AlcalaF G Prendergast
Oct 6, 1985·Nature·R W SchevitzP B Sigler
Aug 21, 1995·FEBS Letters·C CléryP Masson
Dec 1, 1994·Protein Science : a Publication of the Protein Society·K E Neet, D E Timm
Jul 12, 1994·Biochemistry·X PengJ L Silva
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·D Foguel, J L Silva
Jan 1, 1993·Annual Review of Physical Chemistry·J L Silva, G Weber
Jun 1, 1993·Protein Science : a Publication of the Protein Society·J L Silva, C F Silveira
Jun 1, 1993·Proteins·D T Haynie, E Freire
Jan 1, 1995·Methods in Enzymology·C R Robinson, S G Sligar
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Lundbäck, T Härd
Apr 1, 1996·Current Opinion in Structural Biology·J L SilvaP E Prevelige
Feb 1, 1996·Protein Science : a Publication of the Protein Society·Y K MokM Bycroft
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·D FoguelJ L Silva
Nov 14, 1997·Journal of Molecular Biology·C R RobinsonR T Sauer

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Citations

Jul 9, 1999·Biotechnology and Bioengineering·D FoguelA S Robinson
Sep 27, 2000·Biochimica Et Biophysica Acta·S E BondosJ Jonas
May 2, 2002·Biochimica Et Biophysica Acta·Jerson L SilvaDébora Foguel
May 4, 2000·Protein Science : a Publication of the Protein Society·Y K MokG de Prat-Gay
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·L M LimaJ L Silva
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·R Mohana-BorgesG de Prat-Gay
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·A D Ferrão-GonzalesD Foguel
Mar 27, 2007·Protein Science : a Publication of the Protein Society·Diana E WetzlerGonzalo de Prat-Gay
Sep 22, 2015·Biochemistry·Arianna CeccarelliGiampiero Mei
Aug 10, 2004·Biophysical Journal·Daniella IshimaruJerson L Silva
Jan 16, 2003·FEBS Letters·M Laura CeruttiFernando A Goldbaum
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Marek IngrJan Konvalinka
Sep 14, 2004·The Journal of Biological Chemistry·Luis Maurício T R Lima, Jerson L Silva
Mar 27, 1999·Journal of Molecular Biology·P C de SousaD Foguel

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