Characterization of a pepper mild mottle tobamovirus strain capable of overcoming the L3 gene-mediated resistance, distinct from the resistance-breaking Italian isolate

Molecular Plant-microbe Interactions : MPMI
Shinya TsudaYuichiro Watanabe

Abstract

Green pepper plants with the L3 resistance gene usually develop necrotic lesions on leaves infected with a Japanese strain of pepper mild mottle tobamovirus (PMMoV-J). A recently discovered strain, PMMoV-Ij, has the ability to overcome L3 resistance. Phytopathological responses of a variety of plant species to PMMoV-J and PMMoV-Ij were determined and the coat protein (CP) sequence comparisons revealed both amino acids 43 and 50 of PMMoV-Ij were unique. This led us to believe that substitutions at these residues would enable PMMoV-J to overcome L3 resistance. This was confirmed by Western blot (immunoblot) detection of PMMoV-J containing both point mutations in upper uninoculated leaves of resistant plants. Computer models suggest the critical residues in overcoming resistance lie in CP regions that putatively interact with other subunits. These results contribute to our understanding of the virus's ability to circumvent plant resistance.

References

Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T SaitoY Okada
Nov 11, 1986·Nucleic Acids Research·M IshikawaY Okada
Jan 1, 1984·Analytical Biochemistry·D A Knecht, R L Dimond
Jan 1, 1997·Molecular Plant-microbe Interactions : MPMI·A de la CruzI García-Luque
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A Knorr, W O Dawson

❮ Previous
Next ❯

Citations

Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W O Dawson
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·M A EscobarA M Dandekar
May 12, 2012·Molecular Plant Pathology·Hiroyuki MizumotoYasufumi Hikichi
Oct 1, 2010·The Journal of General Virology·Carmelo LópezLuis Rubio
Jul 1, 2003·Plant Disease·Kazumi SuzukiJun Murai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.