PMID: 9418240Jan 7, 1998Paper

Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi

FEMS Microbiology Letters
C Pissavin, Nicole Hugouvieux-Cotte-Pattat

Abstract

The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthetic substrate, confirmed that rotA encodes a periplasmic peptidyl-prolyl cis-trans isomerase. rotA disruption provokes no change in cell morphology, cell viability, growth rate or stability of the extracellular and periplasmic proteins. In addition, this mutation does not alter the activity of the pectate lyases, their stability in the periplasm during the transitory step of secretion or their recognition by the Out secretory system. rotA expression was followed using a rotA::uidA transcriptional fusion. Some environmental conditions, such as temperature variations and nitrogen starvation, modulate rotA expression. In contrast to the E. coli rotA gene, the E. chrysanthemi rotA possesses only one promoter and is not controlled by the CRP global regulator.

References

Dec 1, 1992·Journal of Bacteriology·N Hugouvieux-Cotte-PattatJ Robert-Baudouy
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N P Cianciotto, B S Fields
Jan 1, 1995·Trends in Biochemical Sciences·K E RuddP E Rouviere
Jun 1, 1994·Molecular Microbiology·C Wülfing, A Plückthun
Mar 1, 1996·Journal of Bacteriology·S W Lazar, R Kolter
Dec 1, 1996·Journal of Bacteriology·C PissavinN Hugouvieux-Cotte-Pattat

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Citations

Sep 11, 2017·Journal of Molecular Microbiology and Biotechnology·Aggeliki SkagiaMaria Dimou
Aug 10, 2017·World Journal of Microbiology & Biotechnology·Maria DimouPanagiotis Katinakis
Jan 26, 2007·Molecular Plant-microbe Interactions : MPMI·Ning ZangJi-Liang Tang

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