PMID: 9528672Apr 7, 1998Paper

Characterization of a pH-sensitive surfactant, dodecyl-2-(1'-imidazolyl) propionate (DIP), and preliminary studies in liposome mediated gene transfer

Biochimica Et Biophysica Acta
E Liang, J Hughes

Abstract

The inefficiency of non-viral gene delivery systems, relative to viral systems, is likely due, in part, to the failure of endosomes to release DNA before reaching degradative lysosomes. A solution is to incorporate compounds in a delivery vector that will selectively increase the release of endosomally encapsulated DNA. To meet the above criteria, we designed, synthesized, and characterized the physicochemical and biological properties of such a compound, dodecyl-2-(1'-imidazolyl) propionate (DIP) to enhance cationic liposome mediated gene delivery. Several surface active techniques were used to characterize DIP lysing membranes. The critical micelle concentration of DIP was between 0.10-0.18 mM and the effective release and solubilization ratios were 1.0 and 4.0, respectively. DIP facilitated membrane disruption in both a pH and concentration dependent manner. In the presence of esterase at pH 7.0, the hydrolysis rate increased 32-fold indicating DIP can be degraded in the biological milieu. Toxicity of DIP by MTT assay in the SKnSH cell line demonstrated an ID50 of 1.2 mM, which is 30-fold higher than the concentration of DIP used to enhance gene transfection. When incorporated into cationic-liposomes, DIP enhanced transgene ...Continue Reading

References

Sep 1, 1979·Journal of Medicinal Chemistry·R A FirestoneR J Bonney
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Aug 1, 1990·Pharmaceutical Research·C J ChuF C Szoka
Oct 2, 1989·Biochimica Et Biophysica Acta·P PinnaduwageL Huang
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LemaitreB Lebleu
Jan 13, 1988·Biochimica Et Biophysica Acta·J RuizA Alonso
Jan 1, 1986·Cancer Chemotherapy and Pharmacology·S P Cole
Dec 19, 1985·Biochimica Et Biophysica Acta·D Lichtenberg
Sep 27, 1972·Nature: New Biology·A TrouetC De Duve
Sep 15, 1974·Biochemical Pharmacology·C de DuveF Van Hoof
Sep 14, 1982·Biochemistry·M L JacksonA D Albert
Aug 1, 1981·Analytical Biochemistry·E De VendittisV Bocchini
Jul 17, 1995·Biochimica Et Biophysica Acta·J Lasch
Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
Aug 22, 1994·FEBS Letters·L Carrasco
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·H E HoflandS M Sullivan
May 1, 1992·Trends in Cell Biology·S Akhtar, R L Juliano

❮ Previous
Next ❯

Citations

Nov 1, 2002·Biochemical and Biophysical Research Communications·Hiroyuki KamiyaHideyoshi Harashima
Oct 24, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·E LiangJ A Hughes
Nov 20, 1998·Current Opinion in Biotechnology·D SchermanJ Crouzet
Sep 26, 2012·AAPS PharmSciTech·Orapan PaecharoenchaiPraneet Opanasopit
Nov 3, 2010·Nanomedicine·Inmaculada PosadasValentín Ceña
Apr 12, 2002·Journal of Pharmaceutical Sciences·Aravind Asokan, Moo J Cho
Jan 3, 2012·International Journal of Pharmaceutics·Stefano Salmaso, Paolo Caliceti
Jun 28, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Aravind Asokan, Moo J Cho
Aug 31, 2012·Journal of Drug Targeting·Orapan PaecharoenchaiPraneet Opanasopit
May 31, 2011·Biochimie·Xiao-Xiang ZhangMark W Grinstaff
Jun 13, 2014·Pharmaceutical Biology·Saeed GhanbarzadehSanam Arami
Nov 6, 2013·Journal of Liposome Research·Xin GuoFrancis C Szoka
May 30, 2015·Colloids and Surfaces. B, Biointerfaces·Ali Dehshahri, Hossein Sadeghpour
Apr 20, 2016·The Journal of Physical Chemistry. B·Batoul SrourPetra Hellwig
Sep 1, 1999·The Journal of Pharmacy and Pharmacology·C R Dass, M A Burton
Jul 6, 2016·Chembiochem : a European Journal of Chemical Biology·Philippe PierratLuc Lebeau
Mar 28, 2003·Biochimica Et Biophysica Acta·Aravind Asokan, Moo J Cho
Mar 21, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Aurora ColomerLourdes Pérez
Dec 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaonong ChenRobert Pelton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.