PMID: 2118799Jul 17, 1990Paper

Characterization of a photosystem I core containing P700 and intermediate electron acceptor A1

Biochemistry
P V WarrenJ H Golbeck

Abstract

A new photosystem I core has been isolated that is devoid of the bound iron-sulfur clusters but preserves electron flow from P700 to the intermediate electron acceptor A1. The particle is prepared by incubation of a Synechococcus sp. PCC 6301 photosystem I core protein (which contains electron acceptors A0, A1, and FX) with 3 M urea and 5 mM K3Fe(CN)6 to oxidatively denature the FX iron-sulfur cluster to the level of zero-valence sulfur. In this apo-FX preparation, over 90% of the flash-induced absorption change at 820 nm decays with a 10-microseconds half-time characteristic of the decay of the P700 triplet state formed from the backreaction of P700+ with an acceptor earlier than FX. Chemical reduction at high pH values with aminoiminomethanesulfinic acid results in kinetics identical with those seen in the P700 chlorophyll a protein prepared with sodium dodecyl sulfate (SDS-CP1, which contains only electron acceptor A0); the flash-induced absorption change decays primarily with a 25-ns half-time characteristic of the backreaction between P700+ and A0-, and the magnitude of the total absorption change is larger than can be accounted for by the P700 content alone. Addition of oxygen results in a reversion to the 10-microseconds...Continue Reading

References

Feb 28, 1989·Biochimica Et Biophysica Acta·K G ParrettJ H Golbeck
Jan 1, 1987·Biochimica Et Biophysica Acta·J H Golbeck

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Citations

Jul 30, 2003·The Journal of Biological Chemistry·J Nathan HendersonKevin Redding
Mar 1, 2008·The Journal of Physical Chemistry. B·Shana L BenderBridgette A Barry
Aug 14, 2019·The Journal of Physical Chemistry. B·Oleg G PoluektovLisa M Utschig
Oct 9, 2003·Journal of the American Chemical Society·Bernard MungeJames F Rusling

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