Characterization of a plasminogen activator and its inhibitor in human mesangial cells

Kidney International
R LacaveJ D Sraer

Abstract

In the course of some pathological and experimental nephropathies, intraglomerular fibrin deposits develop, possibly as a consequence of inefficient fibrinolysis. In vitro human glomeruli exhibit fibrinolytic activity due to the synthesis of plasminogen activators (PAs) such as, tissue-type PA (t-PA) and urokinase-type PA (u-PA). Immunofluorescence studies have previously shown that t-PA is localized in the capillary tufts and u-PA in the visceral epithelial cells. We have now investigated the fibrinolytic activity of cultured human mesangial cells. Inhibitory activity towards u-PA or t-PA but not plasmin was found in both conditioned medium and cellular extracts. Analysis of the conditioned medium by zymography revealed a single band of PA-activity (Mr: 110 to 120 kDa). Immunoneutralization with anti-t-PA and anti-plasminogen activator inhibitor (PAI-1) IgG but not anti-u-PA or anti-PAI-2 removed this band. Reverse fibrin autography demonstrated the presence of PAI-1 in both cellular extracts and in conditioned medium. Western Blot analysis showed that two bands (50 kD and 120 kD) were recognized by the anti-PAI-1 antibody. By ELISA t-PA and PAI-1 antigens were found to increase progressively with time in the culture medium bu...Continue Reading

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Citations

Mar 1, 1992·Journal of Cellular Physiology·C J HeE Rondeau
Jan 17, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Pierina De MuroGian Mario Cherchi
May 7, 1999·Kidney International·M Kitamura, L G Fine
Jan 12, 2002·Kidney International·Wolfgang EberhardtJosef Pfeilschifter
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Nov 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·J Mason
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Mar 16, 2011·Circulation·Yacine BoulaftaliMarie-Christine Bouton
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Chi Young SongHyun Soon Lee
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Oct 10, 1998·Kidney International·M KashoM Shichiri
Dec 25, 2003·Thrombosis Research·Hiromi HagiwaraToyohiko Ariga

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