Characterization of a polyubiquitin gene in T. thermophila and of ubiquitin gene expression during sexual reproduction and under stress conditions

Gene
P Guerreiro, C Rodrigues-Pousada

Abstract

A 5-unit polyubiquitin gene, TTU3, was isolated from a T. thermophila genomic library and sequenced. This gene presents an extra triplet coding for Phe, a AGAGA motif and a putative HSE element in its 5'-non-coding region. The ubiquitin gene expression in this ciliate was investigated by Northern blot hybridization in conjugating cells or cells under stress conditions. Exponentially growing cells express two ubiquitin mRNAs of 0.75 and 1.8 kb and a new species of 1.4 kb is induced under hyperthermic stress. During sexual reproduction of the cells (conjugation) the 1.8-kb mRNA is still transcribed whereas the steady-state population of the 0.75 mRNA transcripts is strongly diminished. Southern blot analysis suggests that ubiquitin in T. thermophila constitutes a large family of about ten members.

References

Jan 1, 1992·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jun 13, 1991·Biochimica Et Biophysica Acta·S JentschH P Hauser
Jul 1, 1991·Chromosoma·L J HauserD E Olins
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·A M NevesC Rodrigues-Pousada
Jan 25, 1990·Nucleic Acids Research·C F Brunk, L A Sadler
Feb 11, 1990·Nucleic Acids Research·A NevesC Rodrigues-Pousada
Nov 25, 1990·Nucleic Acids Research·L A StargellM A Gorovsky
Jan 1, 1989·The Journal of protozoology·D W Martindale
Apr 27, 1989·Nature·W R Brown
Jan 1, 1980·Annual Review of Genetics·M A Gorovsky
Jul 1, 1982·Experimental Cell Research·D W MartindaleP J Bruns
Apr 1, 1993·The Biochemical Journal·A J Rivett
Jun 1, 1994·Microbiological Reviews·D M Prescott
May 2, 1994·FEBS Letters·H KrebberT Bakker-Grunwald
Nov 1, 1987·Trends in Ecology & Evolution·P M Sharp, W H Li

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Citations

Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M A ArandaA J Maule
Jan 10, 2003·Molecular Biology and Evolution·John M ArchibaldPatrick J Keeling
Jun 12, 2012·European Journal of Protistology·Xihan LiuJun Gong
Aug 28, 2020·Journal of Virology·Mengmeng ZhaoZhen Lin

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