Characterization of a promiscuous cadmium and arsenic resistance mechanism in Thermus thermophilus HB27 and potential application of a novel bioreporter system

Microbial Cell Factories
Immacolata AntonucciGabriella Fiorentino

Abstract

The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differently from other microbes, are not organized into a single operon. They encode the proteins: arsenate reductase, TtArsC, arsenic efflux membrane transporter, TtArsX, and transcriptional repressor, TtSmtB. In this work we show that the arsenic efflux protein TtArsX and the arsenic responsive transcriptional repressor TtSmtB are required to provide resistance to cadmium. We analyzed the sensitivity to Cd(II) of mutants lacking TtArsX, finding that they are more sensitive to this metal than the wild type strain. In addition, using promoter probe reporter plasmids, we show that the transcription of TtarsX is also stimulated by the presence of Cd(II) in a TtSmtB-dependent way. Actually, a regulatory circuit composed of TtSmtB and a reporter gene expressed from the TtarsX promoter responds to variation in Cd(II), As(III) and As(V) concentrations. Our results demonstrate that the system composed by TtSmtB and TtArsX is responsible fo...Continue Reading

References

Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Emilia PedoneGabriella Fiorentino
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
May 5, 2009·Toxicology and Applied Pharmacology·Lars Järup, Agneta Akesson
Oct 1, 2009·Chemical Reviews·Zhen MaDavid P Giedroc
May 7, 2010·Nucleic Acids Research·Gary MacindoeDavid W Ritchie
Dec 21, 2010·Applied and Environmental Microbiology·Laura AlvarezJosé Berenguer
Apr 3, 2012·Applied and Environmental Microbiology·Se-In ChoeDonald C Sheppard
May 29, 2012·Journal of Bacteriology·Lici A Schurig-Briccio, Robert B Gennis
Jan 2, 2013·Frontiers in Bioscience (Landmark Edition)·Simonetta BartolucciEmilia Pedone
Jun 27, 2013·Biochimica Et Biophysica Acta·Immacolata Del GiudiceGabriella Fiorentino
Dec 10, 2013·Trends in Biochemical Sciences·Kelvin WongIan D Kerr
Apr 15, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Aaron T SmithAmy C Rosenzweig
May 6, 2014·Frontiers in Physiology·Sanjoy Paul, W Scott Moye-Rowley
Sep 16, 2014·Frontiers in Pharmacology·Rajendra Prasad, Manpreet K Rawal
Jan 15, 2015·Biosensors·Jian Chen, Barry P Rosen
Jul 21, 2015·Nature Chemical Biology·Aaron T SmithAmy C Rosenzweig
Jan 16, 2016·Environmental Science and Pollution Research International·Sandra PrévéralDaniel Garcia
May 22, 2016·Applied and Environmental Microbiology·Matilde FernándezTino Krell
Dec 22, 2016·Biochemistry·Aaron T SmithAmy C Rosenzweig
Mar 2, 2017·Analytical Biochemistry·Yuvaraj RavikumarHyungdon Yun
Mar 28, 2017·Nature Reviews. Microbiology·Pete ChandrangsuJohn D Helmann
Jul 12, 2017·Microbial Biotechnology·Immacolata AntonucciGabriella Fiorentino
Dec 1, 2017·Analytical and Bioanalytical Chemistry·Hyun Ju KimSang Jun Lee
Dec 15, 2017·Environmental Science and Pollution Research International·Preeti Ranawat, Seema Rawat

❮ Previous
Next ❯

Citations

Apr 17, 2020·International Journal of Environmental Research and Public Health·Rosanna PuopoloGabriella Fiorentino
Aug 7, 2020·Antioxidants·Emilia PedoneDanila Limauro
Feb 1, 2019·World Journal of Microbiology & Biotechnology·Martina AulittoPatrizia Contursi
Dec 2, 2020·The Science of the Total Environment·Lijin AnMingshun Li
Feb 6, 2021·Scientific Reports·Rosanna PuopoloGabriella Fiorentino
Apr 13, 2021·World Journal of Microbiology & Biotechnology·Syed Azmal AliGurjeet Kaur
Apr 27, 2021·Frontiers in Microbiology·Martina AulittoGabriella Fiorentino
Jul 30, 2019·International Journal of Biological Macromolecules·Giovanni GalloGabriella Fiorentino
Jun 3, 2021·International Journal of Environmental Research and Public Health·Giovanni GalloGabriella Fiorentino

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
biosensor
biosensing
PCR
electrophoretic mobility shift

Software Mentioned

TASSER
Blastall
Hex Protein Docking server

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.