Characterization of a putative 3-deoxy-D-manno-2-octulosonic acid (Kdo) transferase gene from Arabidopsis thaliana

Glycobiology
Martial SévenoPatrice Lerouge

Abstract

The structures of the pectic polysaccharide rhamnogalacturonan II (RG-II) pectin constituent are remarkably evolutionary conserved in all plant species. At least 12 different glycosyl residues are present in RG-II. Among them is the seldom eight-carbon sugar 3-deoxy-d-manno-octulosonic acid (Kdo) whose biosynthetic pathway has been shown to be conserved between plants and Gram-negative bacteria. Kdo is formed in the cytosol by the condensation of phosphoenol pyruvate with d-arabinose-5-P and then activated by coupling to cytidine monophosphate (CMP) prior to its incorporation in the Golgi apparatus by a Kdo transferase (KDTA) into the nascent polysaccharide RG-II. To gain new insight into RG-II biosynthesis and function, we isolated and characterized null mutants for the unique putative KDTA (AtKDTA) encoded in the Arabidopsis genome. We provide evidence that, in contrast to mutants affecting the RG-II biosynthesis, the extinction of the AtKDTA gene expression does not result in any developmental phenotype in the AtkdtA plants. Furthermore, the structure of RG-II from the null mutants was not altered and contained wild-type amount of Rha-alpha(1-5)Kdo side chain. The cellular localization of AtKDTA was investigated by using las...Continue Reading

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Citations

Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chijun LiChristian R H Raetz
Nov 26, 2010·Glycobiology·Magali AudryChristelle Breton
Apr 28, 2010·Plant Physiology·Zoë A Popper, Maria G Tuohy
Mar 19, 2013·The Arabidopsis Book·Yonghua Li-BeissonJohn Ohlrogge
Mar 5, 2011·Annual Review of Plant Biology·Maor Bar-Peled, Malcolm A O'Neill
Mar 5, 2013·Annual Review of Plant Biology·Melani A AtmodjoDebra Mohnen
Apr 17, 2012·Organic & Biomolecular Chemistry·Gulden Camci-UnalNicola L B Pohl
Feb 23, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Raimund HaarmannEnrico Schleiff
Feb 26, 2021·Journal of Mass Spectrometry : JMS·Trevor T ForsmanYoung Jin Lee

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