Characterization of a putative novel type of oligodendrocyte in cultures from rat spinal cord

The European Journal of Neuroscience
M L Fanarraga, E A Milward

Abstract

Oligodendrocytes originate in different neural tube domains, within boundaries of expression of a series of patterning genes which condition the diverse morphogenetic programme of each area. Although neuronal and astrocyte heterogeneity are widely accepted, and despite accumulating evidence for oligodendrocyte heterogeneity in vivo, oligodendrocytes in vitro are currently considered as a homogeneous cell population. The present investigation demonstrates that oligodendrocyte diversity can be detected in vitro and characterizes a novel morphological class of O4-positive oligodendrocyte which is consistently identifiable in rat central nervous system cultures. These cells have a very characteristic epithelioid, unbranched and often lobulated morphology which enables their identification within 2 h of plating. Immunostaining shows that this morphological type is sometimes positive for GD3, A2B5 and vimentin, and most of the time positive for Ranscht antibody, O1 and Rip but negative for glial fibrillary acidic protein, OX-42, neuron-specific enolase, nestin and erbB2. The apparent levels and/or distributions of (i) microtubules, (ii) surface glycolipids recognized by O4, O1 and Ranscht antibody, and (iii) the less specific marker ...Continue Reading

References

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Citations

Aug 23, 2005·Brain Research. Brain Research Reviews·Fernando de Castro, Ana Bribián

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