Characterization of a putative NsrR homologue in Streptomyces venezuelae reveals a new member of the Rrf2 superfamily

Scientific Reports
John MunnochMatthew I Hutchings


Members of the Rrf2 superfamily of transcription factors are widespread in bacteria but their functions are largely unexplored. The few that have been characterized in detail sense nitric oxide (NsrR), iron limitation (RirA), cysteine availability (CymR) and the iron sulfur (Fe-S) cluster status of the cell (IscR). In this study we combined ChIP- and dRNA-seq with in vitro biochemistry to characterize a putative NsrR homologue in Streptomyces venezuelae. ChIP-seq analysis revealed that rather than regulating the nitrosative stress response like Streptomyces coelicolor NsrR, Sven6563 binds to a conserved motif at a different, much larger set of genes with a diverse range of functions, including a number of regulators, genes required for glutamine synthesis, NADH/NAD(P)H metabolism, as well as general DNA/RNA and amino acid/protein turn over. Our biochemical experiments further show that Sven6563 has a [2Fe-2S] cluster and that the switch between oxidized and reduced cluster controls its DNA binding activity in vitro. To our knowledge, both the sensing domain and the putative target genes are novel for an Rrf2 protein, suggesting Sven6563 represents a new member of the Rrf2 superfamily. Given the redox sensitivity of its Fe-S clu...Continue Reading


Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B Grimm
Mar 1, 1990·Journal of General Microbiology·M A GlazebrookL C Vining
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Sep 15, 1996·The Biochemical Journal·H D YounS O Kang
Aug 3, 1999·The EMBO Journal·J G KangJung-Hye Roe
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 10, 2002·Journal of Bacteriology·Ji-Sook HahnJung-Hye Roe
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
Feb 13, 2003·Biochemistry·Gregory J GattoJeremy M Berg
Apr 23, 2003·Archives of Biochemistry and Biophysics·Alan R TessonN G J Richards
May 24, 2003·The Journal of Biological Chemistry·Heather K LambAlastair R Hawkins
Sep 13, 2003·The EMBO Journal·Dimitris Brekasis, Mark S B Paget
Sep 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gregory L Challis, David A Hopwood
Jan 20, 2006·Molecular Microbiology·Matthew I HutchingsMark J Buttner
Jan 28, 2006·Journal of Inorganic Biochemistry·Paul R GardnerJohn S Olson
May 24, 2006·The Journal of Biological Chemistry·Jason C CrackAndrew J Thomson
Apr 25, 2007·Nucleic Acids Research·Warren A Kibbe
Sep 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jinzhong LinJinfeng Wang
Nov 10, 2007·Environmental Microbiology·Geertje van KeulenR Gary Sawers
Apr 24, 2008·Molecular Microbiology·Gerald L Newton, Robert C Fahey
Dec 17, 2008·Nature Reviews. Microbiology·Klas Flärdh, Mark J Buttner
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jul 17, 2010·Molecular Microbiology·Fabiano L ThompsonAVIB Group
Feb 10, 2012·Journal of Natural Products·David J Newman, Gordon M Cragg
Feb 22, 2012·Free Radical Biology & Medicine·Michael T Forrester, Matthew W Foster
May 23, 2012·Archives of Biochemistry and Biophysics·Surabhi Mishra, James Imlay
Aug 29, 2012·Journal of Applied Microbiology·C Feehily, K A G Karatzas
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bo ZhangMichael K Johnson
Dec 21, 2013·Microbial Cell Factories·Héctor RodríguezRamón I Santamaría
Mar 29, 2014·BMC Bioinformatics·Fabian AmmanSven Findeiß
Aug 8, 2014·PLoS Genetics·Mahmoud M Al-BassamMark J Buttner
Feb 3, 2015·Biochimica Et Biophysica Acta·Joana A SantosSandra Macedo-Ribeiro
Sep 20, 2015·Genes & Development·Rohit RejaB Franklin Pugh
Feb 19, 2016·The Journal of Biological Chemistry·Jason C CrackNick E Le Brun
Feb 26, 2016·Scientific Reports·Yasuyuki SasakiHirofumi Shoun


Apr 21, 2017·Nature Communications·Anne VolbedaJuan C Fontecilla-Camps
Oct 3, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Apr 6, 2018·Chemical Science·Ma Teresa Pellicer MartinezNick E Le Brun
Dec 9, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Aug 12, 2020·Journal of Industrial Microbiology & Biotechnology·Yongjae LeeByung-Kwan Cho
Jun 21, 2019·Antibiotics·Thomas C McLeanRebecca Devine

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Bacterial Proteins
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