PMID: 6261228Feb 11, 1981

Characterization of a R plasmid-associated, trimethoprim-resistant dihydrofolate reductase and determination of the nucleotide sequence of the reductase gene

Nucleic Acids Research
J W Zolg, U J Hänggi

Abstract

The trimethoprim-resistant dihydrofolate reductase associated with the R plasmid R388 was isolated from strains that over-produce the enzyme. It was purified to apparent homogeneity by affinity chromatography and two consecutive gel filtration steps under native and denaturing conditions. The purified enzyme is composed of four identical subunits with molecular weights of 8300. A 1100 bp long DNA segment which confers resistance to trimethoprim was sequenced. The structural gene was identified on the plasmid DNA by comparing the amino acid composition of the deduced proteins with that of the purified enzyme. The gene is 234 bp long and codes for 78 amino acids. No homology can be found between the deduced amino acid sequence of the R388 dihydrofolate reductase and those of other prokaryotic or eukaryotic dihydrofolate reductases. However, it differs in only 17 positions from the enzyme associated with the trimethoprim-resistance plasmid R67.

References

Apr 1, 1988·Antimicrobial Agents and Chemotherapy·R de GrootA L Smith
Jan 1, 1987·Antimicrobial Agents and Chemotherapy·L SundströmO Sköld
Mar 12, 1984·Nucleic Acids Research·C Alff-Steinberger
Feb 1, 1995·Antimicrobial Agents and Chemotherapy·P HuovinenO Sköld
Feb 13, 2008·Antimicrobial Agents and Chemotherapy·Carlos RevillaFernando de la Cruz
Feb 24, 2006·Biotechnology and Bioengineering·Michael D Lynch, Ryan T Gill
Feb 12, 1991·Biochemistry·R M BritoP R Rosevear
Aug 2, 2005·Journal of Computer-aided Molecular Design·Hernán AlonsoJill E Gready
Feb 1, 1986·Journal of Biomolecular Structure & Dynamics·V BrendelE N Trifonov

Citations

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C P GrayH Schaller
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, D Gillespie
Mar 15, 1977·European Journal of Biochemistry·P H SeeburgH Schaller
Jul 5, 1977·Journal of Molecular Biology·P T Barth, N J Grinter
May 1, 1978·Nucleic Acids Research·H OhmoriA M Maxam
Oct 1, 1979·Molecular & General Genetics : MGG·G PeterH G Zachau
Nov 1, 1979·Cell·M PechH G Zachau
Jul 1, 1978·Nucleic Acids Research·P J GreeneH W Boyer
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D Pribnow
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
May 20, 1974·Biochemical and Biophysical Research Communications·S G Amyes, J T Smith
Feb 19, 1980·Biochemistry·A A KumarJ H Freisheim

Related Concepts

Alkalescens-Dispar Group
SDS-PAGE
Amino Acids, I.V. solution additive
Trimethoprim
R388
Gel Chromatography
Plasmid R67
Structural gene
Tetrahydrofolate Dehydrogenase
Base Sequence

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