Characterization of a region upstream of exon I.1 of the human CYP19 (aromatase) gene that mediates regulation by retinoids in human choriocarcinoma cells

Endocrinology
T SunE R Simpson

Abstract

The biosynthesis of estrogens is catalyzed by aromatase P450 (P450arom), the product of the CYP19 gene. The tissue-specific expression of the CYP19 gene is regulated by means of tissue-specific promoters through the use of alternative splicing mechanisms. Thus, transcripts containing various 5'-untranslated termini are present in ovary, brain, adipose stromal cells, and placenta. Sequence corresponding to untranslated exon I.1 is present uniquely in 5'-termini of transcripts expressed in human placenta and choriocarcinoma cells, as a consequence of expression driven by a distal promoter, I.1. The goal of the present study was the identification of regulatory elements in this promoter region. Various deletion mutations of the upstream flanking region of exon I.1 were constructed using the PCR or restriction enzyme digestion. The genomic fragments were fused upstream of the luciferase reporter gene. These constructs were transfected into human choriocarcinoma (JEG3) cells. The longest construct employed, -924/+10 bp, expressed the highest luciferase reporter gene activity. The -64/+10 bp and -125/+10 bp constructs showed no reporter gene expression. Transfection of the -201/+10 bp construct resulted in reporter gene expression, b...Continue Reading

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Citations

Jan 30, 2007·The Journal of Steroid Biochemistry and Molecular Biology·David BarreraLorenza Díaz
Mar 18, 2004·Molecular and Cellular Endocrinology·Colin D ClyneEvan R Simpson
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·T YanaseH Nawata
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·J VanselowM Schwerin
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·A Kamat, C R Mendelson
Aug 14, 2001·Journal of Cellular Biochemistry·C M HansenL Binderup
Jun 9, 2012·Reproductive Toxicology·Ewelina HonkiszAnna K Wojtowicz
Jan 26, 2011·Biochimica Et Biophysica Acta·Johan LundqvistKjell Wikvall
Sep 4, 2007·Toxicology Letters·Yun Wang, Lai K Leung
Nov 29, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nadia QuignotEmmanuel Lemazurier
Sep 17, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ewelina Honkisz, Anna K Wójtowicz
Jan 20, 2017·Reviews in Endocrine & Metabolic Disorders·Giovanna MuscogiuriFrancesco Orio
Feb 23, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Nancy Noyola-MartínezDavid Barrera
Aug 31, 2019·Cell Biology International·Thádia Evelyn de AraújoIdessania Nazareth da Costa
Jun 9, 2005·Molecular Endocrinology·Tsuyoshi NakanishiKeiichi Tanaka
May 30, 2020·Physiological Research·M Grzesiak
Oct 16, 1999·European Journal of Biochemistry·R LiangR V Anthony
Nov 5, 1999·The Journal of Biological Chemistry·K YamadaT Okazaki
Apr 3, 2002·The Journal of Biological Chemistry·Colin D ClyneEvan R Simpson

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