PMID: 8962115Dec 10, 1996Paper

Characterization of a replication-incompetent adenovirus type 5 mutant deleted for the preterminal protein gene

Proceedings of the National Academy of Sciences of the United States of America
Jerome SchaackS J Langer

Abstract

An adenovirus type 5 mutant deleted for the preterminal protein (pTP) gene was constructed using cell lines that express pTP. The pTP deletion mutant virus is incapable of replicating in the absence of complementation and does not express detectable levels of viral mRNAs that are expressed only after the onset of replication. Accumulation of early-region mRNAs, including that for E1A, exhibits a lag relative to that observed from the wild-type virus. However, E1A mRNA accumulation attains a steady-state level similar to the level of expression during the early phase of infection with the wild-type virus. In 293-pTP cells (human embryonic kidney cells that express pTP in addition to high levels of adenovirus E1A and E1B proteins), the pTP deletion mutant virus replicates efficiently and yields infectious titers within 5-fold of that of the wild-type virus. The deletion of 1.2 kb of pTP-encoding sequence increases the size of foreign DNA that can be introduced into the virus and, with an absolute block to replication, makes this virus an important tool for gene therapy.

References

Apr 1, 1977·American Journal of Epidemiology·J P FoxM K Cooney
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L RaoE White
Feb 1, 1992·Immunology and Cell Biology·A Müllbacher
Jan 1, 1986·Annual Review of Genetics·A J Berk
Jan 1, 1986·Advances in Virus Research·S J Flint
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D H Weinberg, G Ketner
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1984·Current Topics in Microbiology and Immunology·T Shenk, J Williams
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·K MitaniS Kochanek
Jul 1, 1995·Journal of Virology·J SchaackD Ornelles
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F EngelhardtJ M Wilson
Feb 11, 1994·Science·M ChalfieD C Prasher

❮ Previous
Next ❯

Citations

Jul 8, 1998·Progress in Neurobiology·W T Hermens, J Verhaagen
Nov 14, 1997·Current Opinion in Biotechnology·I KovesdiT J Wickham
Jun 25, 2010·Journal of Virology·Yange ZhangLinda R Gooding
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jerome SchaackJohn Moorhead
May 1, 2019·The Journal of Gene Medicine·Alexandra Krüger-HaagStefan Kochanek

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.