PMID: 7032590Dec 14, 1981Paper

Characterization of a respiratory mutant of Escherichia coli with reduced uptake of aminoglycoside antibiotics

Biochimica Et Biophysica Acta
M E MuirB J Wallace

Abstract

A strain of Escherichia coli (NSW77) which is partially resistant to streptomycin was isolated by selecting for growth on plates supplemented with 12.5 micrograms/ml streptomycin, a concentration which completely inhibits growth of wild-type strains. The low-level resistance of the mutant appears to result from a reduced ability to accumulate streptomycin intracellularly. In addition, the mutant strain is unable to use succinate for growth because of a defective respiratory chain. Thus, membranes of the mutant strain were found to have approximately half the NADH and D-lactate oxidase activity of the parent strain. Moreover, membranes of the mutant were found to contain demethyl-menaquinone and, in place of ubiquinone, a structural analogue, 2-octaprenyl-3-methyl-6-methoxy-1,4 benzoquinone. The mutation responsible for both the Suc-phenotype and partial resistance to streptomycin was found to be located near minute 15 on the bacterial chromosome. Both the biochemical and genetic evidence suggests the the mutation in strain NSW77 resides in the ubi F gene. Another previously characterized ubi F strain was also found to have a reduced capacity to take up an aminoglycoside antibiotic (gentamicin). These results suggest that the re...Continue Reading

References

Mar 15, 1976·The Biochemical Journal·S J Gutowski, H Rosenberg
Jul 7, 1977·Biochimica Et Biophysica Acta·B J Wallace, I G Young
May 16, 1978·European Journal of Biochemistry·J V Höltje
Apr 25, 1978·Molecular & General Genetics : MGG·S H ThorbjarnardóttirG Eggertsson
Jun 1, 1976·Antimicrobial Agents and Chemotherapy·L E Bryan, H M Van den Elzen
Oct 11, 1974·Nature·K Andry, R C Bockrath
Jul 1, 1972·Journal of Bacteriology·R P Holmes, R R Russell
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·P Traub, M Nomura
Dec 1, 1970·Genetical Research·L M Roberts, E C Reeve
Aug 9, 1972·Biochimica Et Biophysica Acta·D W DeamerA R Crofts
Jul 1, 1950·Journal of Bacteriology·B D DAVIS, E S MINGIOLI

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Citations

Jan 15, 2013·Journal of Bacteriology·Gary BaisaRodney A Welch
Nov 8, 2014·Nucleic Acids Research·Rohan BalakrishnanKurt Fredrick
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·R Bentley
Oct 27, 2017·The Journal of Antibiotics·Yoshiaki Takahashi, Masayuki Igarashi
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Aug 1, 2009·EcoSal Plus·R Meganathan, Ohsuk Kwon
Dec 1, 1987·Microbiological Reviews·H W TaberA S Arrow
Nov 1, 2012·ACS Chemical Biology·Bernd Becker, Matthew A Cooper

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