PMID: 12773632May 30, 2003Paper

Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system

Plant & Cell Physiology
Ki-Hong JungGynheung An

Abstract

We have previously generated a large pool of T-DNA insertional lines in rice. In this study, we screened those T-DNA pools for rice mutants that had defective chlorophylls. Among the 1,995 lines examined in the T2 generation, 189 showed a chlorophyll-deficient phenotype that segregated as a single recessive locus. Among the mutants, 10 lines were beta-glucuronidase (GUS)-positive in the leaves. Line 9-07117 has a T-DNA insertion into the gene that is highly homologous to XANTHA-F in barley and CHLH in Arabidopsis: This OsCHLH gene encodes the largest subunit of the rice Mg-chelatase, a key enzyme in the chlorophyll branch of the tetrapyrrole biosynthetic pathway. In the T2 and T3 generations, the chlorina mutant phenotypes are co-segregated with the T-DNA. We have identified two additional chlorina mutants that have a Tos17 insertion in the OsCHLH gene. Those phenotypes were co-segregated with Tos17 in the progeny. GUS assays and RNA blot analysis showed that expression of the OsCHLH gene is light inducible, while TEM analysis revealed that the thylakoid membrane of the mutant chloroplasts is underdeveloped. The chlorophyll content was very low in the OschlH mutants. This is the first report that T-DNA insertional mutagenesis c...Continue Reading

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Citations

Apr 19, 2011·Molecules and Cells·Min-Jung HanGynheung An
Jun 14, 2005·Plant Molecular Biology·Sichul LeeGynheung An
Jul 16, 2005·Plant Molecular Biology·Kodiveri M GothandamYong-Yoon Chung
Nov 23, 2006·Plant Molecular Biology·Yue-Ie HsingSu-May Yu
Oct 9, 2007·Plant Molecular Biology·Su-May YuTuan-Hua David Ho
Aug 23, 2008·Plant & Cell Physiology·Sunok MoonGynheung An
Jan 8, 2009·Plant Physiology·Arjun KrishnanAndy Pereira
Sep 6, 2005·The Plant Cell·Ki-Hong JungGynheung An
Jan 31, 2009·BMC Genomics·Katina Lazarow, Stephanie Lütticke
Sep 10, 2008·Yi chuan = Hereditas·Jing-Tao HuShi-Gui Li
Jul 10, 2009·Yi chuan = Hereditas·Dian-Yi ShiWei-Wei Jin
Oct 13, 2009·Yi chuan = Hereditas·Xiang-Qian ZhangRui-Zhen Zeng
Aug 22, 2014·Plant Cell Reports·Zhiming FengJianmin Wan
Dec 3, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jing XuZuhua He
Jun 26, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Lijing WangBaoshen Liu
Aug 14, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yu PengSheng Teng
Oct 8, 2011·Journal of Plant Physiology·Myoung Ryoul ParkKarl H Hasenstein
Feb 23, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Changhui SunChengcai Chu
May 15, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Tao ChenCailin Wang
Feb 20, 2007·Journal of Plant Physiology·Fenghua WangJiakui Zheng
Oct 21, 2005·The Plant Journal : for Cell and Molecular Biology·Lene Heegaard MadsenJens Stougaard

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