Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit

Scientific Reports
C Carrasco-OrellanaRaul Herrera

Abstract

Fragaria chiloensis is a strawberry endemic from Chile with attractive white-pink fruit, pleasant aroma and taste. However, this fruit has a limited post-harvest period due to fast softening. Several transcription factors (TFs) are involved in the regulation of fruit ripening, and members of the NAC family have been implicated in cell wall remodeling. FcNAC1 was isolated from F. chiloensis fruit, coding a protein of 332 amino acid residues and displaying a characteristic NAC domain at the N terminus. FcNAC1 protein showed nuclear localization. An increase in transcript level was observed during ripening. A sequence of 1488 bp of FcNAC1 promoter was obtained. In silico analysis identified cis elements able to respond to some hormones and Secondary wall NAC binding elements (SNBE), and responding to auxin and ABA. A structural model of FcNAC1 provided evidence for interaction with DNA sequences containing SNBE, while a dual luciferase assay confirmed the transcriptional activation by FcNAC1 of the promoter of FcPL, a gene involved in cell wall remodeling in F. chiloensis fruit. The results suggest the participation of FcNAC1 during ripening development of strawberry fruit, by regulating pectin metabolism during softening.

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Citations

Apr 20, 2019·Journal of Experimental Botany·Xuemin MaBernd Mueller-Roeber
Jun 4, 2019·Frontiers in Plant Science·Maria Alejandra Moya-LeónRaul Herrera
Mar 20, 2019·Food Research International·Luis Morales-Quintana, Patricio Ramos
Feb 25, 2021·The Plant Cell·Carmen Martín-PizarroDavid Posé
May 29, 2021·BMC Plant Biology·Sara ForlaniSimona Masiero

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Datasets Mentioned

BETA
X74755
X74756
AKC96459.1
EU155163.1
KP966107.1
KC527025
KC527027

Methods Mentioned

BETA
PCR
Confocal microscopy

Software Mentioned

MEGA5
ExPASy
SPSS
MODELLER
NLS
PROCHECK
MEGA
BLAST
SMART
ProSA

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