PMID: 9161879Mar 1, 1997Paper

Characterization of a series of vacuolar type H(+)-ATPase inhibitors on CTL-mediated cytotoxicity

Immunology Letters
K TogashiK Nagai

Abstract

Four vacuolar type H(+)-ATPase (V-ATPase) inhibitors, i.e. concanamycin A (CMA), bafilomycin A1 (BMA), destruxin E (DRE) and prodigiosin 25-C (PRG) profoundly blocked the perforin-dependent cytotoxicity mediated by CD8+ CTL clone. Cytoplasmic acidic compartments were not detected under fluorescent microscopy after treatment of the cells with these V-ATPase inhibitors. In the lytic granule fractions, BMA, CMA, DRE and PRG completely abrogated the perforin activity, although these drugs slightly decreased the granzyme A activity. Under the same conditions, BMA and CMA markedly reduced the perforin content, while DRE and PRG had no significant effects as assayed by immunoblotting using anti-perforin antibody. These data suggest that perforin is predominantly inactivated even without proteolysis in DRE- or PRG-treated cells. We propose that acidic pH is essential to maintain not only quantity but also quality of perform in the lytic granules.

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Citations

Jul 6, 2006·Applied Microbiology and Biotechnology·Yerra Koteswara RaoYew-Min Tzeng
Feb 28, 2002·Phytochemistry·M Soledade C PedrasDale E Ward
Mar 31, 2000·Immunology Today·F ButtgereitM D Brand
Jul 4, 2012·Arthritis Research & Therapy·Cornelia M SpiesFrank Buttgereit
Nov 29, 2007·Future Microbiology·Neil R WilliamsonGeorge P C Salmond
Nov 15, 2011·Biotechnology Advances·Bing-Lan Liu, Yew-Min Tzeng
Sep 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mark M PainterKathleen L Collins
Jul 10, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shibani Mitra-KaushikMasanori Terajima
Jul 6, 2018·Cancer Immunology, Immunotherapy : CII·Ruben LacroixChristian U Blank
Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jooeun BaeHans G Klingemann
Feb 6, 2002·Chembiochem : a European Journal of Chemical Biology·A FürstnerK Nagai

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