Characterization of a serologically atypical Shigella flexneri Z isolated from diarrheal patients in Bangladesh and a proposed serological scheme for Shigella flexneri

PloS One
Mohammad ShahnaijKaisar A Talukder

Abstract

Atypical Shigella flexneri Z variant, that agglutinate with E1037 group factor specific monoclonal antisera against Shigella flexneri IV-I but not with other group or type specific antisera, has continuously being isolated in Bangladesh since 1997. Later this serotype has been reported in Indonesia, China and Argentina. Despite being a provisional serotype, continuous isolation of these strains in diverse geographical regions implicated a great necessity to study the overall characteristics of these strains. Therefore, we extensively characterized S. flexneri Z strains using various phenotypic and molecular tools. Of 3569 S. flexneri isolated between 1997 and 2015, 95 strains were identified as S. flexneri Z using a panel of polyvalent absorbed antisera and monoclonal antisera of S. flexneri (MASF). Of them, randomly selected 65 strains were molecular O-serotyped using multiplex PCR and characterized using different phenotypic and molecular techniques (i.e.biotyping, plasmid profile, virulence marker and PFGE) to determine relationship with other subserotypes of S. flexneri. All these atypical S. flexneri Z strains were agglutinated with MASF B and IV-I antisera. Concordantly, these strains were positive to opt-gene, responsibl...Continue Reading

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Citations

Jun 4, 2019·Human Vaccines & Immunotherapeutics·Louis-Antoine Barel, Laurence A Mulard
May 1, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Sina ArabshahiAbbas Ali Imani Fooladi
Jul 28, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Júlia Cunha GonzalesJuliana Pfrimer Falcão
May 1, 2021·Tropical Medicine and Infectious Disease·Abel F N D PhiriGunnar Skov Simonsen

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BioNumerics

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