PMID: 9185168Jul 1, 1997Paper

Characterization of a serotonin receptor in the cnidarian Renilla koellikeri: a radiobinding analysis

Neurochemistry International
I Hajj-Ali, Michel Anctil

Abstract

Serotonin (5-HT) binding sites in membrane preparations from the sea pansy Renilla koellikeri were identified using [3H]5-HT as radioligand and unlabelled 5-HT as displacer of specific binding. Saturation and kinetic studies revealed a mixed population of [3H]5-HT binding sites which as a whole displayed slow association kinetics, saturability, negligible dissociation and low affinity. The dopaminergic antagonist trifluoperazine specifically displaced the non-dissociated binding sites, allowing the characterization of a homogenous population of saturable sites exhibiting faster association and full dissociation. Scatchard analysis of this population revealed a KD of 23-34 nM and binding site density (Bmax) of 8.5-20.6 pmol/mg protein depending on body region. The pharmacological profile of the dissociable [3H]5-HT binding sites could not be categorized with that of any of the existing vertebrate and invertebrate 5-HT receptor subtypes in the current nomenclature. Especially noteworthy was the absolute inability of LSD, a ligand of choice for all hitherto identified invertebrate 5-HT receptors, to compete for the binding sites in sea pansy membrane preparations. It is proposed that these binding sites represent an evolutionary f...Continue Reading

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Citations

Apr 3, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A J Tierney
May 18, 2000·Biochemical and Biophysical Research Communications·D C NewJ T Wong
Apr 21, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Michel Anctil
Jul 7, 2012·Regulatory Peptides·Cornelis J P Grimmelikhuijzen, Frank Hauser
Apr 16, 2013·Peptides·Fabiano Pinheiro da SilvaIrineu Tadeu Velasco

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