PMID: 6972358Nov 15, 1980Paper

Characterization of a serum factor stimulating the differentiation of myelomonocytic leukemic cells

International Journal of Cancer. Journal International Du Cancer
A W Burgess, D Metcalf

Abstract

Culture of WEHI-3B myelomonocytic leukemic cells in semi-solid agar medium containing serum from mice injected with endotoxin serum (ES) led to the development of maturing granulocytes and macrophages in most leukemic colonies. ES contains high levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), a regulator known to stimulate differentiation of these leukemic cells, but an antiserum which neutralized greater than 85% of the GM-CSF in ES did not suppress the differentiation-inducing activity of ES on WEHI-3B cells. The active factor in endotoxin serum stimulating differentiation in WEHI-3B leukemic cells (GM-DF) was separated from most of the GM-CSF by gel filtration using Ultrogel AcA44. The residual CSF associated with the GM-DF appeared to stimulate selectively granulocytic colonies. Disproportionation of GM-DF and GM-CSF was observed in ES fractions obtained using concanavalin-A/Sepharose chromatography: none of the GM-DF bound to this matrix, whereas 40% of the GM-CSF bound and was eluted with competing alpha methylglucopyranoside. Although no separation of GM-CSF and GM-DF was obtained using DEAE-Sepharose, non-isoelectric focusing in amphoteric buffers indicated charge differences between the differentiat...Continue Reading

References

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B Weiss, L Sachs
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Lotem, L Sachs
Nov 15, 1979·International Journal of Cancer. Journal International Du Cancer·D Metcalf
Oct 1, 1975·Journal of Cellular Physiology·R K Shadduck, D Metcalf
Feb 1, 1972·Biulleten' eksperimental'noĭ biologii i meditsiny·K V Il'inZh Zh Spure
Oct 1, 1970·Journal of Cellular Physiology·Y Ichikawa
Feb 15, 1980·International Journal of Cancer. Journal International Du Cancer·D Metcalf
Jun 15, 1980·International Journal of Cancer. Journal International Du Cancer·J LotemL Sachs

❮ Previous
Next ❯

Citations

Apr 1, 1989·Trends in Pharmacological Sciences·G MorstynJ Cebon
Apr 4, 2013·Cytokine & Growth Factor Reviews·Nicos A Nicola
Sep 5, 2006·Nature Clinical Practice. Rheumatology·Jo L EylesIan P Wicks
Aug 10, 2002·The Journal of Trauma·Richard GamelliElisabeth Hahn
Jun 11, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yuan-Chang ChangJing-Gung Chung
Jul 12, 2005·The Journal of Clinical Investigation·Armin SchneiderWolf-Rüdiger Schäbitz
Feb 10, 2010·Experimental & Translational Stroke Medicine·Jens MinnerupWolf-Rüdiger Schäbitz
Aug 23, 2012·PloS One·Gustavo Ferreira Simões, Alexandre Leite Rodrigues de Oliveira
Dec 1, 1986·Cell Biophysics·L Sachs
Nov 21, 2013·Cell and Tissue Research·Anne SchusterKarl-Herbert Schäfer
Jan 1, 1996·Upsala Journal of Medical Sciences·M Höglund
Dec 2, 2004·Expert Opinion on Biological Therapy·Rami S Komrokji, Gary H Lyman
Jan 1, 1989·Cancer Investigation·G MorstynJ E Layton
Mar 24, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Shih-Chieh YuChin-San Liu
Jul 1, 1992·Current Problems in Cancer·G D Demetri
Oct 5, 2010·Environmental Toxicology·Tung-Yuan LaiJing-Gung Chung
Feb 1, 1992·Archives of Biochemistry and Biophysics·B B Aggarwal, E Pocsik
Nov 1, 1982·Analytical Biochemistry·P C Cooper, A W Burgess
Dec 15, 1983·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Jul 15, 1983·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Jan 1, 1987·International Journal of Cell Cloning·N A Nicola
Aug 1, 1982·Journal of Cellular Physiology·N A Nicola, D Metcalf
Feb 15, 1982·International Journal of Cancer. Journal International Du Cancer·D LiebermannL Sachs
Apr 1, 1982·Journal of Cellular Physiology·G Symonds, L Sachs
Jan 15, 1985·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Sep 15, 1981·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Mar 15, 1983·International Journal of Cancer. Journal International Du Cancer·J M HeardB Varet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.