PMID: 7524978Jul 1, 1994Paper

Characterization of a serum factor that decreases albumin mRNA in cultured hepatocytes

In Vitro Cellular & Developmental Biology. Animal
D E Johnston, D M Jefferson

Abstract

When primary cultures of hepatocytes are exposed to media containing fetal bovine serum (FBS) there is a rapid decrease in levels of tissue-specific mRNAs such as albumin mRNA. We used Northern blot analysis to examine mRNA levels in cultured hepatocytes, and characterized the factor in FBS that significantly reduces the steady state albumin mRNA level. Neonatal bovine serum or serum derived from platelet-poor calf plasma proved as potent as did FBS, but commercial bovine serum albumin did not exhibit this inhibitory activity. Inhibitory activity of FBS was not removed by moderate heat treatment, dialysis, or extraction with organic solvents. However, incubation of FBS with a highly anionic detergent such as 0.1% sodium dodecyl sulfate or N-lauroyl sarcosine, followed by extensive dialysis, resulted in sera that did not inhibit expression of albumin mRNA. These sera supported cell attachment and seemed non-toxic toward the cells. Ammonium sulfate fractionation of FBS showed the activity was present in the 45 to 70% fraction, and trypsin digestion destroyed the inhibitory activity. Gel exclusion chromatography gave a molecular weight of 60,000 to 70,000. Fractionation of serum proteins by DEAE-Sephacel or Cibacron blue-agarose s...Continue Reading

References

Jan 1, 1976·Analytical Biochemistry·J H Waite, C Y Wang
Jan 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K K Ericson, T J Yang
Sep 14, 1990·Biochemical and Biophysical Research Communications·N BussoA Guillouzo
Mar 1, 1986·Journal of Steroid Biochemistry·C J Conti, D R Tasat
Sep 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F GouletO Morin
Apr 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N SawadaH C Pitot
Feb 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J C DunnR G Tompkins
Sep 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J J Maher
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A Ben-Ze'evS R Farmer
Jan 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D M JeffersonM A Zern
Sep 1, 1984·Molecular and Cellular Biology·D M JeffersonL M Reid
May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L M Reid, D M Jefferson
Jan 1, 1984·Journal of Neuroscience Research·G E DavisS Varon
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R EnatL M Reid
Jan 1, 1983·Developmental Neuroscience·I BarakatM Sensenbrenner
May 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M A ZernD A Shafritz
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W N Harrington, G C Godman
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C B ChildsH L Moses

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Citations

Oct 4, 2008·The Journal of Physiology·Deborah Langrill BeaudoinJonathan B Demb

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