Characterization of a single gene cluster responsible for methylpendolmycin and pendolmycin biosynthesis in the deep sea bacterium Marinactinospora thermotolerans

Chembiochem : a European Journal of Chemical Biology
Junying MaJianhua Ju

Abstract

The nine-membered indolactam antibiotics belong to a small group of antibiotics showing broad biological activities. However, the in vivo genetic engineering of compounds of this type has not been performed. Here we report the identification of a single gene cluster responsible for the biosynthesis of methylpendolmycin and pendolmycin, two members of this family of antibiotics, from the deep sea bacterium Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis and gene inactivation, coupled with metabolite characterization, reveal that MpnB, a nonribosomal peptide synthetase, MpnC, a cytochrome P450, and MpnD, a prenyltransferase, are sufficient to catalyze the biosynthesis of the two antibiotics from L-Ile (or L-Val), L-Trp, and methionine. MpnD is the first identified enzyme that transfers a C5 prenyl unit in a reverse manner to the C-7 position of a Trp-derived natural product.

References

Apr 13, 1979·Science·J H CardellinaR E Moore
Sep 1, 1991·Journal of Natural Products·H H SunD M Sedlock
Oct 1, 1984·Chemical & Pharmaceutical Bulletin·Y HitotsuyanagiT Sugimura
Oct 15, 1998·Nature Genetics·Y ZhangA F Stewart
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·K KanedaH Seto
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
Sep 16, 2004·Journal of the American Chemical Society·Daniel J Edwards, William H Gerwick
Mar 17, 2009·Nature Chemical Biology·Shuibing ChenDouglas Melton
May 2, 2009·International Journal of Systematic and Evolutionary Microbiology·Xin-Peng TianWen-Jun Li
Dec 17, 2009·Journal of Natural Products·Minh U HuynhDaniel J Edwards

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Citations

Jan 11, 2013·Journal of the American Chemical Society·Jeffrey D RudolfC Dale Poulter
Mar 22, 2015·Chemistry & Biology·Lona M Alkhalaf, Katherine S Ryan
May 2, 2014·Chembiochem : a European Journal of Chemical Biology·Yunchang XieJianhua Ju
Jul 29, 2015·Applied Microbiology and Biotechnology·Julia WinkelblechShu-Ming Li
Apr 24, 2015·Antonie van Leeuwenhoek·Min-Jiao LiuWen-Jun Li
May 19, 2018·Organic & Biomolecular Chemistry·Takayoshi Awakawa, Ikuro Abe
Jun 4, 2017·Applied and Environmental Microbiology·Erquan ChenHuan Wang
Jun 16, 2018·The Journal of Antibiotics·Ikuro Abe
Nov 11, 2020·Natural Product Reports·Jeffrey D RudolfZining Li
Nov 30, 2020·Journal of Molecular Biology·Elena OstertagGeorg Zocher
Mar 7, 2021·Journal of Natural Medicines·Takayoshi Awakawa
Mar 30, 2021·Synthetic and Systems Biotechnology·He-Ping Chen, Ikuro Abe
Jun 30, 2019·Biochemistry·Benjamin W Roose, David W Christianson
Dec 2, 2021·Chembiochem : a European Journal of Chemical Biology·Angela H SoeriyadiBrett A Neilan

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