Characterization of a small-cell-lung-carcinoma cell line from a patient with cancer-associated retinopathy

International Journal of Cancer. Journal International Du Cancer
Y YamajiJ Takahara

Abstract

We examined the biologic properties of a small-cell-lung-carcinoma (SCLC) cell line (designated MN-1112) established from a patient with SCLC who showed paraneoplastic retinopathy syndrome. Morphologic and immunocytochemical analyses showed that MN-1112 cells possess features of the classic type of SCLC. MN-1112 cells grew in suspension forming relatively large clumps of cells with a doubling time of 72 hr. By light-microscope examination, the cells were relatively small and had scanty cytoplasm. The cells produced NSE, ACTH and CK (BB isozyme); they also expressed recoverin, a novel photoreceptor protein, detected by Northern-blot and Western-immunoblot analysis using human-recoverin-specific DNA probe and anti-bovine-recoverin polyclonal antibody. This report shows that human recoverin is expressed in cultured SCLC cells. Our results support the hypothesis that, in cancer-associated retinopathy (CAR) patients, auto-immune antibody targeting for ectopic recoverin in SCLC is initially produced and cross-reacts with the retinal protein, resulting in the retinal degeneration that occurs in CAR patients.

References

Aug 31, 1992·Biochemical and Biophysical Research Communications·A MurakamiG Inana
May 29, 1992·Biochemical and Biophysical Research Communications·K OkazakiH Hidaka
Oct 27, 1989·Science·T TakahashiJ D Minna
Dec 7, 1989·The New England Journal of Medicine·S E Kornguth
Jun 1, 1988·Brain : a Journal of Neurology·J H O'NeillJ Newsom-Davis
Aug 30, 1994·Biochemical and Biophysical Research Communications·S KawamuraP Nef
May 7, 1993·Science·J B HurleyL Stryer

❮ Previous
Next ❯

Citations

Apr 7, 2004·The Tohoku Journal of Experimental Medicine·Hiroshi OhguroMitsuru Nakazawa
Aug 24, 2013·Survey of Ophthalmology·Ehsan Rahimy, David Sarraf
Feb 19, 2005·Archivos de bronconeumología·C BidegainJ Vayreda
Apr 20, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Dinu Stanescu-SegallJean-Paul Heligon
Dec 22, 2004·Current Treatment Options in Neurology·Denise M Damek
Mar 22, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Makoto KobayashiHirokuni Taguchi
Jul 16, 2005·Ocular Immunology and Inflammation·Charles E Thirkill
Mar 1, 1997·Ocular Immunology and Inflammation·C E Thirkill

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Arthur S PolansGrazyna Adamus
Archives of Ophthalmology
C E ThirkillJ L Keltner
Lung Cancer : Journal of the International Association for the Study of Lung Cancer
C E Thirkill
© 2021 Meta ULC. All rights reserved