PMID: 417138Apr 1, 1978

Characterization of a soluble factor that specifically suppresses the in vitro generation of cells cytotoxic for syngeneic tumor cells in mice

The Journal of Immunology : Official Journal of the American Association of Immunologists
F TakeiD G Kilburn


A tumor-specific soluble factor found in extracts of thymocytes from mice bearing small P815 tumors has been demonstrated. This factor is capable of significantly suppressing the in vitro generation of syngeneic cells cytotoxic for P815 targets if it is added to culture vessels within the first 30 hr of culture. The suppressive factor eluted from Sephadex G-100 after hemoglobin and was estimated to have a m.w. in the range of 40 to 60,000. Preparative isoelectric focusing of thymic extracts established that the suppressive material has an isoelectric point in the range of pH 4.6 to 4.9. The suppressive activity of extracts could be removed by passage of the material through immunoadsorbent columns prepared from membrane proteins of P815 cells but not by analogous columns prepared by using L1210 membrane proteins. The suppressive material was not removed by its passage through immunoadsorbent columns containing anti-mouse immunoglobulin.

Related Concepts

Molecular Sieve Chromatography
Immunologic Tumoricidal Activities
Therapeutic Immunosuppression
Isoelectric Focusing
Leukemia L1210
Mice, Inbred DBA
Mast-Cell Sarcoma
Thymus Extracts

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