Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.

PLoS Neglected Tropical Diseases
Simone HarderIris Bruchhaus

Abstract

In order to proceed through their life cycle, Leishmania parasites switch between sandflies and mammals. The flagellated promastigote cells transmitted by the insect vector are phagocytized by macrophages within the mammalian host and convert into the amastigote stage, which possesses a rudimentary flagellum only. During an earlier proteomic study of the stage differentiation of the parasite we identified a component of the outer dynein arm docking complex, a structure of the flagellar axoneme. The 70 kDa subunit of the outer dynein arm docking complex consists of three subunits altogether and is essential for the assembly of the outer dynein arm onto the doublet microtubule of the flagella. According to the nomenclature of the well-studied Chlamydomonas reinhardtii complex we named the Leishmania protein LdDC2. This study features a characterization of the protein over the life cycle of the parasite. It is synthesized exclusively in the promastigote stage and localizes to the flagellum. Gene replacement mutants of lddc2 show reduced growth rates and diminished flagellar length. Additionally, the normally spindle-shaped promastigote parasites reveal a more spherical cell shape giving them an amastigote-like appearance. The muta...Continue Reading

References

Jan 1, 1992·Advances in Parasitology·J Alexander, D G Russell
Nov 1, 1988·The Journal of Cell Biology·S M King, G B Witman
Jan 1, 1983·Memórias do Instituto Oswaldo Cruz·T BastardoF Tejero
Jan 1, 1980·Immunological Communications·A PolsonM H van Regenmortel
Jan 1, 1994·Annual Review of Microbiology·D Zilberstein, M Shapira
Jan 1, 1994·Cell Motility and the Cytoskeleton·C J Brokaw
Oct 1, 1993·Molecular and Biochemical Parasitology·A ShankarR G Titus
Oct 1, 1996·Trends in Biochemical Sciences·A Lupas
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·W W Zhang, G Matlashewski
Feb 19, 1998·Nature·P BastinK Gull
Mar 13, 1998·Molecular and Biochemical Parasitology·C SantrichJ H LeBowitz
Jan 1, 1997·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P H KroneJ B Sass
Jun 20, 1998·Cell Motility and the Cytoskeleton·J TuxhornW L Dentler
Mar 9, 1999·Molecular Biology of the Cell·M E PorterW Dentler
Oct 3, 1999·The Journal of Biological Chemistry·E L Snapp, S M Landfear
Dec 15, 2000·Cell Biology International·R A Bloodgood
Feb 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C BogdanR Mertens
Feb 19, 2002·Molecular and Biochemical Parasitology·Phalgun B JoshiW Robert McMaster
Oct 16, 2002·Trends in Parasitology·Emanuela Handman, Denise V R Bullen
Jun 28, 2003·The Journal of Biological Chemistry·Wen-Wei ZhangGreg Matlashewski
Oct 9, 2003·The EMBO Journal·Linda KohlPhilippe Bastin
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Jonathan M Scholey
Nov 26, 2003·International Journal for Parasitology·Martin WieseIris Görcke
May 13, 2004·Molecular and Biochemical Parasitology·Philip G NugentDeborah F Smith
Aug 1, 1996·Parasitology Today·P BastinK Gull
Sep 24, 2004·Nature Reviews. Microbiology·Philippe Desjeux
Jul 7, 2005·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Aug 2, 2005·Molecular and Biochemical Parasitology·Christine AdhiamboJonathan H LeBowitz
Feb 10, 2006·Molecular Biology of the Cell·Maja ErdmannMartin Wiese
Jan 18, 2008·Molecular Biology of the Cell·Che-Chia Tsao, Martin A Gorovsky
May 13, 2008·International Journal for Parasitology·Katherine S Ralston, Kent L Hill
Jul 16, 2008·Current Opinion in Microbiology·Paul A Bates

❮ Previous
Next ❯

Citations

Mar 20, 2014·ELife·Cécile Fort, Philippe Bastin
May 26, 2010·Molecular Microbiology·Laura-Isobel McCall, Greg Matlashewski
Jun 15, 2010·Current Opinion in Microbiology·Eva GluenzPaul G McKean
May 27, 2014·Frontiers in Immunology·Rudra Chhajer, Nahid Ali
Apr 9, 2017·Molecular and Biochemical Parasitology·G Srinivas ReddyChinmoy Sankar Dey

❮ Previous
Next ❯

Datasets Mentioned

BETA
AAK72125
BAB88833

Methods Mentioned

BETA
PECs
PCR
electrophoresis
neomycinphosphotransferase
transfection
FCS
transmission electron microscopy
scanning electron microscopy

Software Mentioned

ImageJ
OpenLab
Improvision

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.