PMID: 7514586Mar 1, 1994Paper

Characterization of a taxol-resistant human small-cell lung cancer cell line

Japanese Journal of Cancer Research : Gann
S OhtaN Saijo

Abstract

Taxol is a novel anticancer agent with activity against a broad range of tumors. It has a unique ability to stabilize polymerized tubulin into microtubule bundles within the cell. We have established a taxol-resistant human small-cell lung cancer cell line (H69/Txl) by exposing H69 cells to stepwise increases in taxol concentration. The resistance of H69/Txl cells to taxol was 4.7-fold that of the original H69 cells: the IC50 values for H69 and H69/Txl were 113.7 +/- 56.54 nM and 538.7 +/- 214.7 nM by the tetrazolium dye assay, respectively. Removal of the drug from the medium resulted in a 38% decrease in the growth rate of H69/Txl as compared with that in the presence of 30 nM taxol, suggesting that the growth of H69/Txl was partially dependent on taxol. H69/Txl showed higher sensitivity to vinca alkaloids such as vindesine, vincristine and vinblastine than the parental H69. There was no significant difference in intracellular [3H]taxol content between H69 and H69/Txl cells. No MDR-1 mRNA was detected in H69/Txl by the reverse transcription polymerase chain reaction. There was no significant difference of total and polymerized tubulin content between H69 and H69/Txl cells. Altered mobility of one of the alpha-tubulin isoforms...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1992·Analytical Biochemistry·T KinoshitaK Shimotohno
May 20, 1992·Journal of the National Cancer Institute·S RaoI Ringel
Feb 1, 1991·Investigational New Drugs·A I EinzigT J Smith
Dec 18, 1991·Journal of the National Cancer Institute·F A HolmesG N Hortobagyi
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K E NoonanI B Roninson
Jan 1, 1990·Cancer Chemotherapy and Pharmacology·K MinatoN Saijo
Jan 24, 1991·Journal of Immunological Methods·D ThrowerL Wilson
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J WolffG Palumbo
Dec 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P F JurankaV Ling
Aug 2, 1989·Journal of the National Cancer Institute·S L LaiA F Gazdar
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S KawasakiF P McCormick
Dec 1, 1985·The Journal of Cell Biology·K GreerJ L Rosenbaum
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B A Eipper
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz
Jul 1, 1983·The Journal of Cell Biology·S W L'Hernault, J L Rosenbaum

❮ Previous
Next ❯

Citations

Jan 1, 1997·Journal of Cancer Research and Clinical Oncology·H AriokaN Saijo
Jun 2, 2007·Cancer Chemotherapy and Pharmacology·Katja WosikowskiMaureen Caligiuri
Aug 1, 1997·General Pharmacology·H Parekh, H Simpkins
Aug 26, 1999·General Pharmacology·S SangrajrangA Fellous
Jun 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P ReineckeC D Gerharz
Jan 28, 2010·Cancer Research·Jason Doles, Michael T Hemann
Oct 25, 2003·Oncogene·George A OrrSusan Band Horwitz
Oct 4, 2006·Expert Opinion on Drug Metabolism & Toxicology·Aalok NadkarSanjay Awasthi
Aug 13, 2013·Expert Opinion on Medical Diagnostics·Eric S Kim, Kishan J Pandya
Oct 3, 2002·Japanese Journal of Cancer Research : Gann·Hideki EnokidaMasayuki Nakagawa
Nov 27, 1995·International Journal of Cancer. Journal International Du Cancer·K NishioN Saijo
Apr 2, 2004·Nature Reviews. Cancer·Mary Ann Jordan, Leslie Wilson
May 28, 2008·British Journal of Haematology·Ramadevi NimmanapalliMelissa Alsina
Apr 3, 1999·Current Opinion in Oncology·K NishioN Saijo
Aug 14, 1999·The Journal of Biological Chemistry·M L Gonzalez-GarayF Cabral
Jul 14, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joseph K ZolnerciksKenneth J Linton
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M MonzóC Martín
Mar 11, 2021·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Tuyelee DasAbhijit Dey
Aug 22, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·F Cabral

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.