Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33.

Microbial Cell Factories
Dongyang LiuQirong Shen

Abstract

Recently, the increased demand of energy has strongly stimulated the research on the conversion of lignocellulosic biomass into reducing sugars for the subsequent production, and β-glucosidases have been the focus because of their important roles in a variety fundamental biological processes and the synthesis of useful β-glucosides. Although the β-glucosidases of different sources have been investigated, the amount of β-glucosidases are insufficient for effective conversion of cellulose. The goal of this work was to search for new resources of β-glucosidases, which was thermostable and with high catalytic efficiency. In this study, a thermostable native β-glucosidase (nBgl3), which is secreted by the lignocellulose-decomposing fungus Aspergillus fumigatus Z5, was purified to electrophoretic homogeneity. Internal sequences of nBgl3 were obtained by LC-MS/MS, and its encoding gene, bgl3, was cloned based on the peptide sequences obtained from the LC-MS/MS results. bgl3 contains an open reading frame (ORF) of 2622 bp and encodes a protein with a predicted molecular weight of 91.47 kDa; amino acid sequence analysis of the deduced protein indicated that nBgl3 is a member of the glycoside hydrolase family 3. A recombinant β-glucosida...Continue Reading

References

Oct 15, 1992·European Journal of Biochemistry·T WatanabeM Kuwahara
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Mar 22, 2000·Journal of Agricultural and Food Chemistry·Z Zheng, K Shetty
Aug 15, 2001·European Journal of Biochemistry·P VäljamäeG Johansson
Jan 1, 1997·Biotechnology Advances·M K Bhat, S Bhat
Dec 1, 1982·Applied and Environmental Microbiology·W E Workman, D F Day
Oct 6, 2006·Applied Microbiology and Biotechnology·Jiong HongHidehiko Kumagai
Jan 1, 2010·Applied Microbiology and Biotechnology·Marimuthu JeyaJung-Kul Lee

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Citations

Nov 12, 2016·FEBS Open Bio·Alicia Hernández-GuzmánJulio C Villagómez-Castro
Jan 17, 2016·International Journal of Biological Macromolecules·Shivangi ChamoliAshok Kumar Verma
Jan 1, 2013·Biomolecules·Annette SørensenBirgitte K Ahring
Oct 12, 2018·Preparative Biochemistry & Biotechnology·Tobias Pereira de MoraisRodrigo Simões Ribeiro Leite
Jul 17, 2020·Biotechnology for Biofuels·Malene B KellerPeter Westh
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Shohreh AriaeenejadGhasem Hosseini Salekdeh
Dec 14, 2017·Chembiochem : a European Journal of Chemical Biology·Veeresh Juturu, Jin Chuan Wu
Apr 25, 2020·Archives of Microbiology·Évelyn T BarrilliSérgio L Alves
Aug 29, 2014·The Journal of Biological Chemistry·Saeid KarkehabadiMats Sandgren
Jul 13, 2018·Microorganisms·Juan-José Escuder-RodríguezMaría-Isabel González-Siso
Jan 9, 2021·Journal of Biomolecular Structure & Dynamics·Anica DadwalTulasi Satyanarayana
Dec 3, 2014·Enzyme and Microbial Technology·Yuhong HuangLene Lange

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Datasets Mentioned

BETA
AAX39011.1
6
HQ836475

Methods Mentioned

BETA
mechanical treatment
PCR
column
column chromatography
gel filtration

Software Mentioned

GraphPad Prism
NetNGlyc
MASCOT
SignalP
DNAMAN
BLAST
GraphPad

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