Characterization of a transient outward K+ current with inward rectification in canine ventricular myocytes

The American Journal of Physiology
G R LiS Nattel

Abstract

The threshold potential for the classical depolarization-activated transient outward K+ current and Cl- current is positive to -30 mV. With the whole cell patch technique, a transient outward current was elicited in the presence of 5 mM 4-aminopyridine (4-AP) and 5 microM ryanodine at voltages positive to the K+ equilibrium potential in canine ventricular myocytes. The current was abolished by 200 microM Ba2+ or omission of external K+ (K+o) and showed biexponential inactivation. The current-voltage relation for the peak of the transient outward component showed moderate inward rectification. The transient outward current demonstrated voltage-dependent inactivation (half-inactivation voltage: -43.5 +/- 3.2 mV) and rapid, monoexponential recovery from inactivation (time constant: 13.2 +/- 2.5 ms). The reversal potential responded to the changes in K+o concentration. Action potential clamp revealed two phases of Ba2(+)-sensitive current during the action potential, including a large early transient component after the upstroke and a later outward component during phase 3 repolarization. The present study demonstrates that depolarization may elicit a Ba2(+)- and K(+o)-sensitive, 4-AP-insensitive, transient outward current with inw...Continue Reading

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Citations

Jun 3, 1999·European Journal of Clinical Investigation·P AukrustS S Frøland
Mar 10, 2000·Journal of Cardiovascular Pharmacology·M BarancikW Schaper
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