Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool

PLoS Neglected Tropical Diseases
Ge Ge SunJing Cui

Abstract

Trichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis is not easy to be made. Trichinella spiralis muscle larva (ML) excretory-secretory (ES) antigens are the most widely applied diagnostic antigens for human trichinellosis, but the major drawback of the ES antigens for assaying anti-Trichinella antibodies is the false negative in the early Trichinella infection period. The aim of this study was to characterize the T. spiralis putative serine protease (TsSP) and to investigate its potential use for diagnosis of trichinellosis. The full-length TsSP sequence was cloned and expressed, and recombinant TsSP (rTsSP) was purified by Ni-NTA-Sefinose Column. On Western blotting analysis the rTsSP was recognized by T. spiralis-infected mouse serum, and the natural TsSP was identified in T. spiralis ML crude and ES antigens by using anti-rTsSP serum. Expression of TsSP was detected at various T. spiralis developmental stages (newborn larvae, muscle larvae, intestinal infective larvae and adult worms). Immunolocalization identified the TsSP principally in cuticles and stichosomes of the nematode. The sensitivity of rTsSP-ELISA and ES-ELISA was 98.11...Continue Reading

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Citations

Sep 30, 2020·Veterinary Research·Xin YueZhong Quan Wang
Dec 5, 2020·Research in Veterinary Science·Jia XuJing Cui

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Methods Mentioned

BETA
ELISA
PCR
acetylation

Software Mentioned

SMART
MEGA
SPSS
BOXSHADE
Expasy
Pepstats
Clustal X
DNAStar

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