Characterization of a tyramine receptor type 2 from hemocytes of rice stem borer, Chilo suppressalis

Journal of Insect Physiology
Shun-Fan WuGong-Yin Ye

Abstract

Calcium acts as a second messenger in many cell types, including insect hemocytes. Intracellular calcium level has a definite role in innate and adaptive immune signaling. Biogenic amines such as octopamine (OA), tyramine (TA), dopamine (DA) and serotonin (5-HT) play various important physiological roles in insects by activating distinct G-protein-coupled receptors (GPCRs) that share a putative seven transmembrane domain structure. OA and 5-HT have been shown that can mediate insect hemocytic immune reactions to infections and invasions. Here, we showed that TA increase hemocyte spreading in the rice stem borer, Chilo suppressalis. Furthermore, we cloned a cDNA encoding a tyramine receptor type 2 from the hemocytes in the C. suppressalis, viz., CsTA2, which shares high sequence similarity to members of the invertebrate tyramine receptor family. The CsTA2 receptor was stably expressed in human embryonic kidney (HEK) 293 cells, and its ligand response has been examined. Receptor activation with TA induced a dose-dependent increase in intracellular Ca(2+) concentration ([Ca(2+)]i) in cells, with an EC50 value of 18.7±5.3 nM, whereas OA, DA, 5-HT and other potential agonists did not have this response. The mRNA is present in variou...Continue Reading

References

Jan 1, 1994·Archives of Insect Biochemistry and Physiology·D Baines, R G Downer
Oct 9, 1999·Progress in Neurobiology·T Roeder
Aug 17, 2002·Neuroscience Letters·Yuki NagayaAkira Komatsu
Sep 13, 2002·Insect Biochemistry and Molecular Biology·M D Lavine, M R Strand
Sep 10, 2004·Annual Review of Entomology·Thomas Roeder
Nov 9, 2005·Biochemical and Biophysical Research Communications·Giuseppe CazzamaliCornelis J P Grimmelikhuijzen
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrew B BarronGene E Robinson
Feb 6, 2008·Current Biology : CB·Susanne C HoyerMartin Heisenberg
Apr 9, 2008·Biochemical and Biophysical Research Communications·Jia HuangYoshihisa Ozoe
Jun 3, 2008·Current Opinion in Immunology·Masatsugu Oh-hora, Anjana Rao
Jul 1, 2008·General and Comparative Endocrinology·Angela B Lange
Dec 18, 2008·Nature Immunology·Monika Vig, Jean-Pierre Kinet
Sep 17, 2009·Immunological Reviews·Masatsugu Oh-hora
Nov 17, 2009·The Journal of Experimental Biology·Edward M Blumenthal
Jul 14, 2010·Journal of Insect Physiology·Heleen VerlindenJozef Vanden Broeck
Oct 19, 2011·Archives of Insect Biochemistry and Physiology·Koigoora SrikanthYonggyun Kim
Jan 30, 2013·Journal of Neurochemistry·Asha BaylissPeter D Evans
Apr 23, 2013·Developmental and Comparative Immunology·Shunfan WuGongyin Ye

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Citations

Feb 1, 2017·BMC Biology·Philipp Bauknecht, Gáspár Jékely
Jun 27, 2018·Pharmacological Reviews·Raul R GainetdinovMark D Berry
Jul 28, 2018·Frontiers in Physiology·Vaishnavi SukumarPäivi H Torkkeli
Mar 11, 2017·Scientific Reports·Haiying Zhang, Edward M Blumenthal
Mar 7, 2020·Journal of Industrial Microbiology & Biotechnology·Xue SuiYu Deng

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