Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus

Journal of Virology
Burtram C FieldingPhuay-Yee Goh

Abstract

A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other CoVs, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122-amino-acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical endoplasmic reticulum (ER) retrieval motif, KRKTE. U122 was expressed in SARS-CoV-infected Vero E6 cells, as it could be detected by Western blot and immunofluorescence analyses. U122 is localized to the perinuclear region of both SARS-CoV-infected and transfected cells and colocalized with ER and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional.

References

Nov 1, 1994·The Journal of Cell Biology·E C GaynorS D Emr
Nov 26, 1997·Journal of Virology·V P Nguyen, B G Hogue
Mar 14, 2003·FEBS Letters·Robert EichlerWolfgang Garten
Mar 29, 2003·Journal of Virology·Bert Jan HaijemaPeter J M Rottier
May 6, 2003·Science·Marco A MarraRachel L Roper
May 16, 2003·The New England Journal of Medicine·Kathryn V Holmes
May 16, 2003·Nature·Ron A M FouchierAlbert D M E Osterhaus
Aug 15, 2003·The Journal of General Virology·Volker ThielJohn Ziebuhr

❮ Previous
Next ❯

Citations

May 28, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Wai S ChanAnthony W I Lo
Feb 26, 2005·Bioinformatics·Daniel Shegogue, W Jim Zheng
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Feb 11, 2010·Virology Journal·Natalia VasilenkoAlexander Zakhartchouk
Nov 21, 2007·Biological Chemistry·Karen Hänel, Dieter Willbold
Aug 19, 2008·Biochimica Et Biophysica Acta·Zhongde YeYong Xie
Nov 30, 2007·Virus Research·Krishna NarayananShinji Makino
Sep 11, 2007·Virus Research·Yong GuoJiang Gu
May 2, 2007·Annals of the New York Academy of Sciences·T Mizutani
Jul 6, 2014·Antiviral Research·Ding Xiang LiuRolf Hilgenfeld
May 25, 2005·Paediatric Respiratory Reviews·David Burgner, Peter Richmond
Feb 15, 2005·Antiviral Research·Yee-Joo TanWanjin Hong
Apr 4, 2006·Biochemical and Biophysical Research Communications·Burtram C FieldingYee-Joo Tan
Dec 20, 2005·The Journal of Pathology·Anthony W I LoKa-Fai To
Jul 28, 2020·Cellular and Molecular Life Sciences : CMLS·Rajanish GiriVladimir N Uversky
Jan 22, 2021·Endocrine Journal·Gábor Speer, Péter Somogyi
May 28, 2021·Journal of Chemical Information and Modeling·Alberto OngaroAlessandra Gianoncelli
Jul 6, 2021·Bioinformatics and Biology Insights·Ritesh GorkhaliHitesh Kumar Bhattarai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.