PMID: 8614629Apr 1, 1996Paper

Characterization of a UV endonuclease gene from the fission yeast Schizosaccharomyces pombe and its bacterial homolog

Nucleic Acids Research
M TakaoA Yasui

Abstract

From the fission yeast Schizosaccharomyces pombe, a cDNA fragment was isolated, which confers UV resistance on repair deficient Escherichia coli host cells. The cloned cDNA encodes a protein of 68,815 Da, which has a 36.6% identity of amino acid sequence with the previously identified 74 kDa UV endonuclease of the filamentous fungus Neurospora crassa. Analysis of several truncated gene constructs shows that only the C-terminal two thirds region, which has 54% identity of amino acid sequence with the C-terminal region of the Neurospora homolog, is necessary for complementing activity of UV-sensitivity in the E. coli host cells. Purified recombinant protein from E. coli host cells incises both UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts at the sites immediately 5' to the DNA damage in the same fashion as the Neurospora protein. Furthermore, a bacterial homologous sequence was isolated from Bacillus subtilis and shows a similar complementing activity of UV sensitivity in E. coli host cells, indicating a wide distribution of this alternative excision repair mechanism in life.

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Citations

Jun 10, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Yasui, S J McCready
Mar 5, 2004·Fungal Genetics and Biology : FG & B·Gustavo H Goldman, Etta Kafer
Aug 1, 2000·Mutation Research·S J McCreadyA Yasui
Aug 1, 1998·Nucleic Acids Research·J L Alleva, P W Doetsch
Aug 14, 1999·Nucleic Acids Research·M LombaertsJ Brouwer
Jun 12, 2003·Nucleic Acids Research·Thierry DoukiJean Cadet
Nov 30, 2005·Nucleic Acids Research·Jennifer A Roberts, Malcolm F White
Feb 26, 2009·Nucleic Acids Research·Lars SchomacherHans-Joachim Fritz
Sep 11, 2012·Journal of Bacteriology·Fernando H Ramírez-GuadianaMario Pedraza-Reyes
May 16, 2003·Molecular and Cellular Biology·Satoshi OkanoAkira Yasui
May 2, 2003·Bioscience, Biotechnology, and Biochemistry·Shigeru KitayamaHiroshi Watanabe
Jan 21, 2009·DNA Repair·Keti PaspalevaNora Goosen
Feb 23, 2007·Annals of Neurology·Makito HiranoSatoshi Ueno
Dec 24, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoru Mochida, Mitsuhiro Yanagida
Mar 1, 1997·Nucleic Acids Research·S DaveyG A Freyer
Oct 17, 2007·Structure·Caroline Kisker
Oct 17, 2007·Structure·Keti PaspalevaJan Pieter Abrahams
Apr 4, 2002·Fungal Genetics and Biology : FG & B·Gustavo H GoldmanSteven D Harris
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Liton Kumar SahaShunichi Takeda
Jun 22, 1999·Molecular and Cellular Biology·B KaurP W Doetsch
Feb 1, 2000·Biochemical and Biophysical Research Communications·M LombaertsJ Brouwer
Nov 7, 1998·Mutation Research·R S Lloyd
Oct 24, 2007·DNA Repair·Nora Goosen, Geri F Moolenaar
Feb 19, 2002·Chemical Reviews·Sheila S. David, Scott D. Williams

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