Characterization of a xenograft model of human ovarian carcinoma which produces intraperitoneal carcinomatosis and metastases in mice

International Journal of Cancer. Journal International Du Cancer
K L MolpusT Hasan

Abstract

A new xenograft model for human epithelial ovarian carcinoma, with extensive intraperitoneal (i.p.) carcinomatosis as the predominant disease manifestation, is described. Cells from the established NIH:OVCAR-5 cell line were injected i.p. into 6- to 8-week-old Swiss nude mice. Comparative analyses between cells cultured in vitro and tumor cells derived ex vivo were performed to assess histologic features, immunohistochemical cell markers, hormonal receptor expression, adhesion to extracellular matrix molecules and chromosomal constitution. Macroscopically, the extent of tumor development appeared to be site-dependent and tumor cell survival was dose-dependent. Advanced disease was characterized by extensive solid tumor burden and ascites with parenchymal invasion, lymphatic metastases and vascular dissemination. Individual tumor nodules exhibited developing neovasculature characterized by the absence of mature basement membrane. Despite some histologic loss of cellular differentiation in advanced disease, antigenic expression was preserved, distinguishing these cells as epithelial in origin. Karyotyping of tumor cells demonstrated multiple numeric and structural chromosomal abnormalities. Serum and ascites CA 125 levels were co...Continue Reading

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Citations

Feb 28, 2014·Proceedings of the National Academy of Sciences of the United States of America·Bryan Q SpringTayyaba Hasan
Mar 10, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·K. MujooN. J. Donato
Dec 6, 2001·The American Journal of Pathology·R C CaseyA P Skubitz
May 4, 2006·Lasers in Surgery and Medicine·Nicolas SolbanTayyaba Hasan
Jun 1, 2013·Gynecologic Oncology·Amy P N SkubitzDaniel A Vallera
Sep 10, 2015·IEEE Transactions on Nanobioscience·Giuliano MazziniSabina Merlo
Apr 2, 2010·Journal of Ovarian Research·Allison C SharrowAllan D Hess
Oct 20, 2005·Journal of the National Cancer Institute·Marcela G del CarmenTayyaba Hasan
Sep 24, 1999·Journal of the National Cancer Institute·L R DuskaT Hasan
Sep 10, 2003·Hematology/oncology Clinics of North America·Gustavo Rodriguez
Oct 20, 2006·Nature·D YelinG J Tearney
Jul 23, 2005·Clinical & Experimental Metastasis·Kathryn M BurlesonAmy P N Skubitz
Mar 21, 1998·Cellular Signalling·J A Buczek-ThomasT Hasan
Feb 7, 2013·Journal of Ovarian Research·Jianjun ZhangHe Wang

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