PMID: 8972846Dec 1, 1996Paper

Characterization of a zinc-dependent transcriptional activator from Arabidopsis

Nucleic Acids Research
S de PaterJ Kijne

Abstract

The C2-H2 zinc-finger is a widely occurring DNA binding motif, usually present as tandem repeats. The majority of C2-H2 zinc-finger proteins that have been studied are derived from animals. Here, we characterize a member of a distinct class of plant C2-H2 zinc-finger proteins in detail. A cDNA clone encoding a DNA binding protein from Arabidopsis was isolated by SouthWestern screening. The protein, termed ZAP1 (Zinc-dependent Activator Protein-1), is encoded by a single copy gene, which is expressed to similar levels in root and flower, to a somewhat lower level in stem and to low levels in leaf and siliques. The optimal binding site was determined by random binding site selection, and the consensus sequence found is CGTTGACCGAG. The homology between ZAP1 and other DNA binding proteins is restricted to a repeated region of a stretch of 24 highly conserved amino acids followed by a zinc-finger motif (C-X4-C-X22-23-H-X1-H). The C-terminal zinc-finger region is essential for DNA binding, whereas deletion of the N-terminal one resulted in 2.5-fold reduced binding affinity. Binding of ZAP1 to DNA was abolished by metal-chelating agents. The activation domain as determined in yeast is adjacent to and possibly overlapping with the DNA...Continue Reading

References

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Citations

Dec 18, 2004·Plant Molecular Biology·Caterina MarèLuigi Cattivelli
Mar 6, 1999·Current Opinion in Plant Biology·P J Rushton, I E Somssich
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Jan 6, 2012·The Journal of Biological Chemistry·Kazuhiko YamasakiShigeyuki Yokoyama
Jan 27, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Kai-Fa WeiDao-Xin Xie
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Monika KaldeBernadette Lippok
May 10, 2002·Genes & Development·Silke Robatzek, Imre E Somssich
Aug 18, 2004·Plant Physiology·Anthony L ContentoDiane C Bassham
Feb 12, 2005·The Plant Cell·Kazuhiko YamasakiShigeyuki Yokoyama
Nov 19, 2009·The Plant Cell·Rajnish KhannaShu-Hsing Wu
Jan 5, 2005·BMC Evolutionary Biology·Yuanji Zhang, Liangjiang Wang
May 21, 2011·BMC Plant Biology·Marcel C van VerkHuub J M Linthorst
Apr 20, 2014·International Journal of Genomics·Hui SongXingjun Wang
Dec 20, 2013·Bioinformatics·Krzysztof PolanskiKatherine J Denby
Jan 24, 2014·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Feng WenZhaoqing Chu
Jan 17, 2002·Bioscience, Biotechnology, and Biochemistry·K MaeoK Nakamura
Mar 17, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Anwar A KhanDing S Shih
Jan 13, 2016·Development Genes and Evolution·Jing JinHaiyang Jiang
Jan 29, 2016·Plant Molecular Biology·Zhu QiaoWei Zhang

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