Characterization of Actinobacillus seminis biofilm formation.

Antonie van Leeuwenhoek
J Fernando Montes GarcíaErasmo Negrete-Abascal

Abstract

Actinobacillus seminis is an autochthonous gram-negative bacterium that affects reproductive organs, causing epididymitis, low fertility, and occasional abortions in ovine and goats. The virulence factors and the pathogenicity mechanisms of A. seminis have not been clearly elucidated yet. In this work, biofilm production by A. seminis in in vitro assays is described and characterized. After 48-h incubation at 37 °C in trypticase soy broth, A. seminis formed biofilms containing an extracellular matrix comprised mainly of fibrillar material. Microaerophilia or the presence of calcium diminished biofilm formation in approximately 50% and 70%, respectively, but low iron concentrations increased it 40%. Through enzymatic digestion, it was found that proteins were the main component of these biofilms. Structural observations through scanning electron microscopy indicated the presence of a high amount of fibrillar material in which bacteria were immersed. Antibodies against different bacterial surface proteins, such as anti-biofilm matrix and anti-adhesin, diminished biofilm formation in 70% and 25%, respectively; whereas furanone C-30 and LED-209, compounds described as quorum-sensing inhibitors, completely inhibited biofilm formatio...Continue Reading

References

Oct 5, 1991·The Veterinary Record·P J HeathI A Aitken
Jun 1, 2000·Veterinary Microbiology·A SchallerJ Frey
Jan 16, 2009·Journal of Clinical Microbiology·Elizabeth Gladys Aarag FredheimJohanna Ericson Sollid
Sep 10, 2009·Veterinary Research·Josée LabrieMario Jacques
Dec 8, 2009·New Zealand Veterinary Journal·W A Al-Katib, S M Dennis
Dec 25, 2012·Colloids and Surfaces. B, Biointerfaces·Sudhir K Shukla, T Subba Rao
Jul 6, 2013·Journal of Molecular Biology·Oscar A McCrateLynette Cegelski
Jan 5, 2014·Nature Reviews. Microbiology·Dennis ClaessenGilles P van Wezel
Mar 5, 2014·TheScientificWorldJournal·Valéria S MoustacasTatiane A Paixão
Nov 13, 2014·MBio·Meredith M CurtisVanessa Sperandio
Nov 28, 2014·Veterinary Research·Bernardo Bello-OrtíMario Jacques
Dec 7, 2014·Veterinary Microbiology·Ismail Boukahil, Charles J Czuprynski
Apr 22, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Lena DewenterBerenike Maier
Jun 25, 2015·Microbiology Spectrum·Jiunn N C Fong, Fitnat H Yildiz
Aug 1, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Ashwin KannanPennathur Gautam
Sep 18, 2015·Archives of Microbiology·Priya GuptaProsun Tribedi
Jan 6, 2016·Current Microbiology·J F Montes-GarcíaE Negrete-Abascal
Feb 9, 2016·Current Topics in Microbiology and Immunology·Briana Petruzzi, Thomas J Inzana
May 15, 2018·Research in Veterinary Science·Monalessa Fábia PereiraDenise Mara Soares Bazzolli
Jun 12, 2018·Current Biology : CB·Anna DragošÁkos T Kovács
Jun 24, 2019·Antonie van Leeuwenhoek·Juan Fernando Montes-GarcíaErasmo Negrete-Abascal

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Methods Mentioned

BETA
scanning electron microscopy
light microscopy

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