Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates

The Open Microbiology Journal
Carolina ArangoLuis E Díaz

Abstract

New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents. An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach. Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS. The results suggest that combination of physicochemical pretreatments and tax...Continue Reading

References

Sep 1, 1976·Applied and Environmental Microbiology·C Hagedorn
Sep 29, 2004·Revista médica de Chile·Sergio LeivaHéctor García-Quintana
Apr 9, 2005·The Journal of Antibiotics·János Bérdy
Jun 9, 2005·Chemistry : a European Journal·Kerstin HeroldChristian Hertweck
Oct 31, 2006·Mycological Research·Maria Elisabete da Silva BarrosJúnia Soares Hamdan
Dec 19, 2007·Progress in Drug Research. Fortschritte Der Arzneimittelforschung. Progrès Des Recherches Pharmaceutiques·Frank E Koehn
Jul 16, 2008·Applied and Environmental Microbiology·Danheng QiuYing Huang
Feb 27, 2009·International Journal of Systematic and Evolutionary Microbiology·Shu-Kun TangWen-Jun Li
Apr 25, 2009·Nature Protocols·Anja Freiwald, Sascha Sauer
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Julian Davies, Dorothy Davies
Jun 28, 2011·Journal of Microbiological Methods·Jonas GhyselinckPaul De Vos
Jan 31, 2012·Journal of Microbiological Methods·E Esin Hameş-Kocabaş, Ataç Uzel
Feb 4, 2012·Indian Journal of Pharmaceutical Sciences·Sonashia Velho-Pereira, N M Kamat
Jun 22, 2012·Antonie van Leeuwenhoek·Katherine CellerGilles P van Wezel
Apr 18, 2013·Journal of Biotechnology·Maria Isabel StetsLeonardo Magalhães Cruz
Jun 26, 2013·International Journal of Antimicrobial Agents·Peter W Taylor
Aug 21, 2013·Microbiological Research·Panchanathan ManivasaganSe-Kwon Kim
Sep 13, 2013·Journal of Natural Products·Jane Y YangPieter C Dorrestein
Sep 24, 2013·Applied Microbiology and Biotechnology·Ramesh Subramani, William Aalbersberg
Jan 7, 2014·Bioorganic & Medicinal Chemistry Letters·Dean G BrownTricia L May-Dracka
Nov 5, 2014·Frontiers in Microbiology·Nadim CassirPhilippe Brouqui
Apr 10, 2015·Marine Drugs·Gabriel Zamith Leal DalmasoAlane Beatriz Vermelho
Nov 27, 2015·Microbiology and Molecular Biology Reviews : MMBR·Essaid Ait BarkaGilles P van Wezel
Apr 19, 2016·Marine Drugs·Juan Pablo Gomez-EscribanoMervyn J Bibb

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

TOF Biotyper
Chromas
RTC Biotyper
MALDI
BioEdit Sequence Alignment Editor
Bruker Flex Biotyper RTC
SILVA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.