Characterization of additional components of the environmental pH-sensing complex in the pathogenic fungus Cryptococcus neoformans

The Journal of Biological Chemistry
Kaila M PianaltoJ Andrew Alspaugh

Abstract

Pathogenic microorganisms must adapt to changes in their immediate surroundings, including alterations in pH, to survive the shift from the external environment to that of the infected host. In the basidiomycete fungal pathogen Cryptococcus neoformans, these pH changes are primarily sensed by the fungus-specific, alkaline pH-sensing Rim/Pal pathway. The C. neoformans Rim pathway has diverged significantly from that described in ascomycete fungi. We recently identified the C. neoformans putative pH sensor Rra1, which activates the Rim pathway in response to elevated pH. In this study, we probed the function of Rra1 by analyzing its cellular localization and performing protein co-immunoprecipitation to identify potential Rra1 interactors. We found that Rra1 does not strongly colocalize or interact with immediate downstream Rim pathway components. However, these experiments identified a novel Rra1 interactor, the previously uncharacterized C. neoformans nucleosome assembly protein 1 (Nap1), which was required for Rim pathway activation. We observed that Nap1 specifically binds to the C-terminal tail of the Rra1 sensor, probably promoting Rra1 protein stability. This function of Nap1 is conserved in fungi closely related to C. neof...Continue Reading

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Citations

Jul 6, 2019·Frontiers in Cellular and Infection Microbiology·Mélissa Caza, James W Kronstad
Jun 18, 2020·MBio·Hannah E BrownJ Andrew Alspaugh
Jul 3, 2021·International Journal of Molecular Sciences·Mahek Momin, Ginny Webb

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