PMID: 8590955Jan 1, 1996Paper

Characterization of adhesion receptors on cultured microvascular endothelial cells derived from the retroorbital connective tissue of patients with Grave's ophthalmopathy

European Journal of Endocrinology
A E Heufelder, P C Scriba

Abstract

T lymphocytes have been demonstrated recently to play an important role in the pathogenesis and propagation of Graves' ophthalmopathy (GO). Recruitment of T cells to the retroorbital tissue in GO involves the activation of certain adhesion molecules both in the vascular endothelium and in the extravascular connective tissue within the retroorbital space. To characterize the interactions between orbital endothelial cells (OECs) and circulating T cells in vitro, we discussed a two-step immunopurification procedure with bead-immobilized Ulex europaeus I lectin and anti-human endothelial cell antigen (CD31) monoclonal antibody for rapid and reproducible isolation of highly pure microvascular endothelial cell populations from small quantities of retroorbital connective tissue. Endothelial origin of the resulting cell populations was confirmed by positive immunoreactivity for von Willebrand factor. CD31 and thrombomodulin. Under baseline conditions, GO-OECs, but not normal OECs, expressed intercellular adhesion molecule 1 (ICAM-1) and CD44 immunoreactivity but no immunoreactivity for endothelial leukocyte adhesion molecule 1 (ELAM-1) and vascular cell adhesion molecule 1 (VCAM-1) was detected. Exposure of GO-OEC and normal OEC monola...Continue Reading

Citations

Apr 9, 1998·Baillière's Clinical Endocrinology and Metabolism·A E Heufelder
Apr 16, 2002·Thyroid : Official Journal of the American Thyroid Association·Tomasz BednarczukJanusz Nauman
Aug 1, 1996·Thyroid : Official Journal of the American Thyroid Association·F G DelemarreH A Drexhage
Jun 12, 1998·Thyroid : Official Journal of the American Thyroid Association·G E Krassas
Feb 24, 2001·Critical Reviews in Clinical Laboratory Sciences·M YamadaJ R Wall
Apr 27, 2000·Endocrine Reviews·L BartalenaC Marcocci
Jul 15, 1998·Medizinische Klinik·G Förster, G Kahaly
May 15, 2021·Journal of Cellular and Molecular Medicine·Ziyi ChenYue Wang

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