Characterization of Adsorbents for Cytokine Removal from Blood in an In Vitro Model

Journal of Immunology Research
Stephan HarmJens Hartmann

Abstract

Cytokines are basic targets that have to be removed effectively in order to improve the patient's health status in treating severe inflammation, sepsis, and septic shock. Although there are different adsorbents commercially available, the success of their clinical use is limited. Here, we tested different adsorbents for their effective removal of cytokines from plasma and the resulting effect on endothelial cell activation. The three polystyrene divinylbenzene (PS-DVB) based adsorbents Amberchrom CG161c and CG300m and a clinically approved haemoperfusion adsorbent (HAC) were studied with regard to cytokine removal in human blood. To induce cytokine release from leucocytes, human blood cells were stimulated with 1 ng/ml LPS for 4 hours. Plasma was separated and adsorption experiments in a dynamic model were performed. The effect of cytokine removal on endothelial cell activation was evaluated using a HUVEC-based cell culture model. The beneficial outcome was assessed by measuring ICAM-1, E-selectin, and secreted cytokines IL-8 and IL-6. Additionally the threshold concentration for HUVEC activation by TNF-α and IL-1β was determined using this cell culture model. CG161c showed promising results in removing the investigated cytokin...Continue Reading

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Citations

Mar 20, 2016·Innate Immunity·Stephan HarmJens Hartmann
Feb 22, 2017·The International Journal of Artificial Organs·Jens Hartmann, Stephan Harm
May 20, 2017·The International Journal of Artificial Organs·Khosrow S HouschyarFrank Siemers

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Methods Mentioned

BETA
size exclusion chromatography
scanning
flow cytometry
FACS

Software Mentioned

FlowJo

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