PMID: 3778423Oct 1, 1986

Characterization of alcohol dehydrogenase in young soybean seedlings

Biochemical Genetics
R BrzezinskiJ P Thirion

Abstract

Molecular properties of alcohol dehydrogenase (ADH) were examined in young soybean seedlings. Soybean radicle tissue is ADH-rich. Enzyme specific activity decreases slowly with the development of roots and becomes almost undetectable when the first true leaves appear. Soybean ADH was not found to be inducible by flooding. 2,4-Dichlorophenoxyacetic acid (2,4-D) treatment increased ADH specific activity as much as 14-fold. Only one ADH isozyme was detected by isoelectric focusing. By DNA-DNA hydridization, soybean ADH genomic sequences were shown to be partly homologous to maize ADH1 cDNA. The presence of more than one Adh gene in soybean is discussed.

References

Feb 11, 1985·Nucleic Acids Research·E S DennisW J Peacock
Jan 1, 1985·Annual Review of Genetics·M Freeling, D C Bennett
Aug 1, 1980·Biochemical Genetics·R J FerlD Schwartz
May 11, 1984·Nucleic Acids Research·E S DennisW J Peacock
Jan 1, 1983·Methods in Enzymology·H C Birnboim
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J K RobertsM Freeling
Jul 1, 1980·Cell·M M SachsR Okimoto
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W L GerlachW J Peacock
Apr 23, 1982·Science·L D Gottlieb

Citations

Dec 1, 1989·Biochemical Genetics·K TihanyiJ P Thirion
Sep 1, 1992·Plant Physiology·R A KennedyT C Fox
Feb 1, 1990·Plant Physiology·D A RussellM M Sachs
Jul 16, 2005·Analytical Biochemistry·Lynn A LittererDavid A Somers

Related Concepts

Alcohol Dehydrogenase I
DNA, Double-Stranded
Enzyme Induction
Glycine max
Monosan

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