PMID: 2500152Jun 13, 1989Paper

Characterization of aldose reductase and aldehyde reductase from rat testis

Biochimica Et Biophysica Acta
N KawasakiA Tanaka

Abstract

Aldose reductase (alditol:NAD(P)+ 1-oxidoreductase, EC 1.1.1.21) and aldehyde reductase (alcohol:NADP+ oxidoreductase, EC 1.1.1.2) were purified to a homogeneity from rat testis. The molecular weights of aldose reductase and aldehyde reductase were estimated to be 38,000 and 41,000 by SDS-polyacrylamide gel electrophoresis, and the pI values of these enzymes were found to be 5.3 and 6.1 by chromatofocusing, respectively. Aldose reductase had activity for aldo-sugars such as xylose, glucose and galactose, whereas aldehyde reductase was virtually inactive for these aldo-sugars. The Km values of aldose reductase for aldo-sugars were relatively high. When a correction was made for the fraction of aldo-sugar present as the aldehyde form, which is the real substrate of the enzyme, the Km values were much lower. Aldose reductase utilized both NADPH and NADH as coenzyme, whereas aldehyde reductase utilized only NADPH. Aldose reductase was activated significantly by sulfate ion, while aldehyde reductase was little affected. Both enzymes were inhibited strongly by the known aldose reductase inhibitors. However, aldehyde reductase was in general less susceptible to these inhibitors when compared to aldose reductase. Both aldose reductase ...Continue Reading

References

Apr 12, 1979·Biochimica Et Biophysica Acta·R A Boghosian, E T McGuinness
Jan 1, 1975·Annual Review of Medicine·K H Gabbay
May 1, 1985·Archives of Biochemistry and Biophysics·B Das, S K Srivastava
Jun 28, 1972·Biochemical and Biophysical Research Communications·R S Clements, A I Winegrad
Dec 1, 1967·Journal of Neurochemistry·G I Moonsammy, M A Stewart
Feb 1, 1983·Biochemical Medicine·H B MarkusH Harris
Mar 1, 1984·Chemical & Pharmaceutical Bulletin·T TanimotoJ Kawamura
Aug 21, 1984·Biochimica Et Biophysica Acta·S K SrivastavaB Das
Jun 1, 1982·Archives of Biochemistry and Biophysics·K InagakiJ Okuda
May 1, 1982·The Histochemical Journal·T S Kern, R L Engerman
Jan 1, 1960·Biochimica Et Biophysica Acta·H G HERS

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Citations

Jan 1, 1991·The International Journal of Biochemistry·T TanimotoT Machida
Jul 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·E PailhouxC Jean
Jan 15, 1995·European Journal of Biochemistry·T GuiC Nishimura
Jan 15, 1996·European Journal of Biochemistry·N IwataT Endo
Jul 1, 1993·The Journal of Clinical Investigation·R R WuT N Palmer
Mar 19, 2013·Future Medicinal Chemistry·Ahmed Mehanna
Jan 1, 1992·Journal of Diabetes and Its Complications·D Dvornik
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·A Bhatnagar, S K Srivastava
May 20, 2003·Molecular & Cellular Proteomics : MCP·Emmanuelle ComCharles Pineau
Apr 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T Nakada, I L Kwee
Mar 6, 2018·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tsuyoshi Tanimoto
Sep 1, 1996·Zeitschrift für Ernährungswissenschaft·D Schumacher, L W Kroh
Apr 1, 1995·Applied and Environmental Microbiology·A KuhnB A Prior
Oct 1, 1990·Archives of Biochemistry and Biophysics·N IwataT Satoh

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