Characterization of allergen (Bet v 1)-specific T cell lines and clones from non-allergic individuals

International Archives of Allergy and Immunology
C EbnerD Kraft

Abstract

The immune response towards allergens in non-allergic healthy individuals was investigated. T cell lines (TCL) with specificity for Bet v 1, the major birch pollen allergen, were established and analysed for epitope specificity. 49 T cell clones (TCC) specific for Bet v 1 were isolated from TCLs. All TCCs revealed the Th phenotype. Cytokine production in response to specific stimulation revealed a majority of Th clones producing interleukin (IL)-4 and interferon (IFN)-gamma; however, most TCCs revealed a low IL-4/IFN-gamma ratio. Immunoblot revealed Bet v 1-specific IgG in non-allergic individuals whereas no IgE could be detected. Our results indicate that T cells from allergic and non-allergic individuals recognize the same epitopes on allergenic molecules, leading to activation, which then results in a differential production of cytokines and consequently to differential isotype switching in allergen-specific B cells.

Citations

Jul 1, 2014·International Immunopharmacology·Timothy J Chapman, Steve N Georas
Apr 30, 2014·International Archives of Allergy and Immunology·Alina NeunkirchnerWinfried F Pickl
Mar 1, 2006·Expert Review of Clinical Immunology·Judith A Woodfolk
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin HimlyFátima Ferreira
Jun 26, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E HolenS Elsayed
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laurence Van OvertveltPhilippe Moingeon
Jan 25, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H JealM Jones

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