PMID: 11524115Aug 29, 2001

Characterization of alpha-galactosidases from germinating soybean seed and their use for hydrolysis of oligosaccharides

Phytochemistry
Valéria Monteze GuimarãesC R Felix

Abstract

Raffinose oligosaccharides (RO) are the major factors responsible for flatulence following ingestion of soybean derived products. Removal of RO from seeds or soymilk would then have a positive impact on the acceptance of soy-based foods. Enzymic hydrolysis of the RO is accomplished by alpha-galactosidase. While the content of RO decreases during seed germination, the activity of alpha-galactosidase increases substantially. Two alpha-galactosidases were isolated from germinating seeds by partition in an aqueous two-phase system followed by ion-exchange and affinity chromatography. One of the enzyme preparations (P1) showed a single protein with M(r) of 33 kDa, and the second (P2) had two proteins with M(r) of 31 and 33 kDa. Maximal activities against the synthetic substrate rho-nitrophenyl-alpha-D-galactopyranoside (rhoNPGal) were detected at pH 5.0-5.5 and 45-50 degrees C. Both enzymes were fairly stable at 40 degrees C, but lost most of their activities after 30 min at 50 degrees C. The K(m) values for hydrolysis of rhoNPGal by the P1 and P2 enzymes were 1.55 and 0.76 mM, respectively. The K(m) values determined for hydrolysis of raffinose and melibiose by the P2 enzyme were 5.53 and 5.34 mM, respectively and galactose was a c...Continue Reading

References

Jul 18, 2006·Planta·Bozena ChrostKarin Krupinska
Feb 26, 2008·Biochemistry. Biokhimii︠a︡·O A SinitsynaA P Sinitsyn
Jun 21, 2006·Plant Foods for Human Nutrition·Lílian da Silva FialhoSebastião Tavares de Rezende
Oct 22, 2008·Applied Biochemistry and Biotechnology·Daniel Luciano FalkoskiSebastião Tavares de Rezende
Feb 19, 2011·Applied Biochemistry and Biotechnology·Joana Gasperazzo FerreiraSebastião Tavares de Rezende
Feb 25, 2011·Carbohydrate Research·Pollyanna A VianaValéria Monteze Guimarães
Jan 20, 2010·International Journal of Biological Macromolecules·Pollyanna A VianaValéria Monteze Guimarães
Oct 7, 2008·Phytochemistry·Lílian da Silva FialhoSebastião Tavares de Rezende
Jul 17, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Dan QiuHenry T Nguyen
May 10, 2013·Journal of Animal Physiology and Animal Nutrition·L TortolaA C Carciofi
Jun 16, 2010·Journal of the Science of Food and Agriculture·Oscar Aguilar, Marco Rito-Palomares
Nov 29, 2005·Plant Physiology and Biochemistry : PPB·Pierre MarracciniVincent Pétiard
Jan 20, 2017·Journal of Industrial Microbiology & Biotechnology·Yingbiao ZhouJuankun Zhang
Jun 14, 2017·Critical Reviews in Food Science and Nutrition·Jing-Kun YanJian-Yong Wu
Apr 12, 2018·Anais Da Academia Brasileira De Ciências·Rafael D BuenoEveraldo G DE Barros

Related Concepts

Thermodynamics
Melibiose
Galactose Measurement
Enzymes, antithrombotic
Glycine max
Seed Germination
2-Mercaptoethanol
Soybean preparation
Galactose
Enzyme agent

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.