Characterization of amino acid side chain losses in electron capture dissociation

Journal of the American Society for Mass Spectrometry
Helen J CooperAlan G Marshall

Abstract

We have used electrospray ionization (ESI) Fourier-transform ion cyclotron resonance (FTICR) mass spectrometry to characterize amino acid side chain losses observed during electron capture dissociation (ECD) of ten 7- to 14-mer peptides. Side-chain cleavages were observed for arginine, histidine, asparagine or glutamine, methionine, and lysine residues. All peptides containing an arginine, histidine, asparagine or glutamine showed the losses associated with that residue. Methionine side-chain loss was observed for doubly-protonated bombesin. Lysine side-chain loss was observed for triply-protonated dynorphin A fragment 1-13 but not for the doubly-protonated ion. The proximity of arginine to a methoxy C-terminal group significantly enhances the extent of side-chain fragmentation. Fragment ions associated with side-chain losses were comparable in abundance to those resulting from backbone cleavage in all cases. In the ECD spectrum of one peptide, the major product was due to fragmentation within an arginine side chain. Our results suggest that cleavages within side chains should be taken into account in analysis of ECD mass spectral data. Losses from arginine, histidine, and asparigine/glutamine can be used to ascertain their pre...Continue Reading

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Citations

May 28, 2003·Mass Spectrometry Reviews·Roman A Zubarev
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Séverine ZirahJean-Claude Tabet
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Qiangwei XiaJoshua J Coon
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Shannon L Cook, Glen P Jackson
Dec 3, 2011·Journal of the American Society for Mass Spectrometry·Tao SongIvan K Chu
Feb 1, 2012·Journal of the American Society for Mass Spectrometry·Neil G RumachikJoshua J Coon
Jun 7, 2012·Journal of the American Society for Mass Spectrometry·Thomas W ChungFrantisek Tureček
Jun 4, 2013·Journal of the American Society for Mass Spectrometry·Małgorzata A Kaczorowska, Helen J Cooper
Aug 7, 2013·Journal of the American Society for Mass Spectrometry·Brian L FreyLloyd M Smith
Jul 6, 2004·Journal of the American Society for Mass Spectrometry·Christopher M AdamsKim F Haselmann
May 26, 2005·Journal of the American Society for Mass Spectrometry·Paul A ChrismanScott A McLuckey
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Oct 29, 2005·Journal of the American Society for Mass Spectrometry·Helen J Cooper
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